ECOLOGICAL STUDIES ON TILE SYMBIOSIS OF TER ITOMYCES ...

Inocula of the fungi were placed on plates of the medium,
incorporating the substance to be tested, as shown in Fig. 3-1-1--
The fungi used were An=. rpillus ni2er, CophalosL2rium sp. ',
Penicillium, spo and a sterile mycelial isolate WPP, In the course
of testing the substances it was reallsed that WIT would not pose
a great problem due to its slow growth and Paecilomyces variottl
was substituted for it*
The plates wero Incubated at 29'OC and the growth of the fungi
recordod after 24 wW 43 hours., If t1le plates ware not overgrown by
this time the experiment was continued for 5 days*
The scale used to record the growth of the fungi from the
inoculum plug towards TrrmitomXces was
GROWTH (cm) SCALE
0 0
0-00-)0.49 1
0.5040.99 2
1-0041.49 3
> x. 49 4
One of the problems in developing selective media In that
substances appearing suitable when incorporated in the medium on
thoir own may be unsuitable when used in combinations This may be
due to antagonism between thmt reducing their affectivenesal or
synergism resulting in an increased inhibition of the fungus being
isolated (Tsao 1970). For this reason various combinations of likely
ingredients were tested an the selectivo medium was developed,
3-1.4.2 Results
The results are given in Table 3.1-4.
83.