ECOLOGICAL STUDIES ON TILE SYMBIOSIS OF TER ITOMYCES ...

3-1-3 CANDIDATE ANTIBACTERIAL, ANTIFIJNGAL AND GROWTH-PMOTING
SUBSTANCES
3-1-3*1 Results
The results are given in Tables 3-1-1 to 3-1*3*
(1) Antibiotics
(a) Chloramphonicol
Chloramphenicol acts by Inhibiting the incorporation of amino
acids into ribosomal proteins (Lukerm 1971). At the
-dose
by the C-Hel, of 0*05 91-1 the growth of Termitomyces was Greatly
reduced (Table 3ol-1) and so this substance was rejected*
(b) Novobiocin
Novobiocin In an antibiotic which prevents synthesis of call
recommended
wall materials In bacterial and in an Inhibitor of protein synthesis*
It also affects bacteria In a variety of other ways Including acting
on the cell membrane (Lukens 1971), It is active against both gram +
and gram - bacterial and also Inhibits the growth of P. Xthium and
? hytophthorae Butler and [line (1958) found that it retarded the
radial growth of fungal colonies and here it reduced the growth of
TemitqmYce-s bY 35% at 0.1 gl-1 (Table 3ol. l)- Duo to this it was
rejected for the selective medium but It was decided to use It In
the general soil fungus medium (sr. ) (Appendix 1) to prevent bacterial
growth* It has the useful advantage that It can be autoclavod.,
(c) Penicillin
Penicillin acts In a similar way to novobiocing preventing
synthesis of cell wall materials In bacteria and inhibiting protein
synthesis
O. c)5 gl-1
(Inkens 1971)o It has been used at 0,0050 0*015# OeO3 and
(Bakerapioel
and Miller 1953), OeO3 gl-l (c. ý1.1. jL968) and
67o