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The evolutionary conservation of the MNI gene was investigated by hybridization of
cDNA probe C (Fig. 3) to genomic DNA, extracted from a number of different species, after
digestion with EcoRI. Hybridization signals were observed in very different species,
including species as evolutionarily distant as Xenopus Laevis and Drosophila Melanogaster
(Fig.S). This result provides evidence that the MNI gene has been highly conserved during
evolution.
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Figure 5.
Zoo blot analysis of EcoRT digested DNA isolated from several species hybridized with MN}
eDNA C. The first 10 lanes wefe exposed for 16 hours and the last 2 lanes for 7 days.
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