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SHORT COMMUNICATION
TABLE 1
Characteristics or 25 Single· Copy Probes ror Chromosome 22
Noll fragment length (kb)
o no. Lab name Size' RFLP HeLa T24
Region I (pler··qll)
D228181 NB17 0.7 BgllllTaql ND~ NRZ'
D228182 NB35 0.8 Bglii ND ND
1)228183 NBS4 1.0 Psil 270 270 + 310·
D228184 NBSS 1.1 360 + 680 + 630 + NRZ NHZ
D22~185 NBI03 0.7 210 + NRZ NRZ
Region n (qll-q12)
D228186 NB14 0.' 1000 1000
D228187 NB20 0.9 1000 1000
0228188 NB21 13 NRZ NHZ
D228189 NB43 1.6 NRZ 670+ NRZ
0228190 NB62 0.6 NHZ NRZ
D22S191 NB66 2.1 190 + NRZ 190+ NIlZ
0228192 NB97 0.7 680 + NRZ NRZ
D22S193 NBI29 0.6 Pst! 265 + 410 + 630 + NRZ 265 + 630 + NRZ
Region lIT (q12-q!.1)
D228194 NB7 0.3 500 500
022819~ NRH 1.1 420 + 500 + NRZ 420 + 500 + NItZ
022~196 NB74 1.6 NHZ NRZ
0228197 NBIOa 0.3 420 + 500 420 + 500
D228198 NB1l6 1.0 760 750
0228199 NUll7 1.9 350 350
0228200 NBil9 1.8 560 560
Begion IV (qI3-qter)
0228201 NB5 0.5 1;,ql 190 ND
0228202 NB8 0.8
NO
ND
D22~203 NB60 1.9
240 + 400 240 + 400
D228204 NB70 0.3
ND
ND
0228205 NB127 0.3 Taql
ND ]50
• ~ize insert in kb.
b ND: not determined.
, NRZ: nonresoll1tion zone.
d Two hyhridiling bands were observed.
peared that markers D2281 (1, 6) and D228186 and
D228187 (both this paper) are located on the same Noll
fragment. The same is presumably the case with 022815
(12) and D228199 (this paper) and with D228195 and
D228197 (both this paper). Comparison of the Noll
fragments of the region centromeric to the Ewing sar·
coma translocation as recently described by Budarfet al.
(2) with those from the same region described here
shows no obvious relations, although it should be mentioned
that especially in region qll-q12 there were very
large Noll fragments that did not resolve under the elec·
trophoresis conditions that we used and are therefore
given as running in the nonresolution zone (sizes o\'er
1 Mb).
Odds
6:t 1.3 . 10~t
nr-I
'"
LJ LJ
26:t 151:t
n
n
206:t 1:1.8
locus: 022St8t 022S163 022S182 02:2St93
Relare-nee
toci:
tOLe 022SI0 seR 022St 022$29
! t
! 1
0.00 004 001
0.12 008
oos! qter
'"
LJ
too.t
FIG. 1. Most likely order and se>:-Ilveraged recombination rates between gene markers On cbromosome 22. Reference lod were used as
anchor points for placing four new markers On the map. The odds against im·ersion of the marker order are shown above or below the brackets
lind lire based on multipoint linkllge aMlyse8. The i()(alization of D228193 is based on physical mapping data.
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