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CHROMOSOME 11 ABERRATIONS
ebellopontine angle and intraspinally. In 1987, several
pamsagittal meningiomas were removed surgically.
The tumors were histologically diagnosed as
fibrous meningiomas (designated ~[N55A). In December
1989, a reCLlrrence of a parasagittal meningioma
on the left side was removed and histologically
diagnosed as a fibrous meningioma (:..tN55B).
Multiple small nodules of hyperplasia were observed
in this region. In October 19l)O, two meningiomas
from the right (:"IN55C) and the left
(i\IN550) cerebellopolltine angle were excised at
an inten'al of 2 weeks; both were of the fibrous
type. The patient's parents have no history of meningiomas.
However, her t~lther (born in 1929) developed
a well-differentiated adenocarcinoma of
the lung in 19H4 and 2 years latera brain metastasis
of this tumor. A CT scan performed in 1990 did not
reveal any intracmnial tumors. When this paper
W,IS written, he was alive ilntl without complaints.
Tissue Culture and Cytogenetic Analysis
The meningioma specimens (MN55B,C,D)
were ohtained within 3{) min after surgery. The
preparation for tissue culture and tissue culture
l'Onditions were as described (Koper et aI., 1990).
Chromosome analysis WilS carried Ollt on cultured
cells after one to three passages using R-(acridine
omnge) and Q-(quinacrine) banding. The karyotypes
were described aCl'onling to ISeN (1991).
DNA Extraction, Southern Blotting,
and Hybridization
High molecular weight DNA was isolated from
I:ultured and fresh tumor ti.~sue and peripheral
blood leucocytes from the patient, her parents, and
unrelated individuals according tu standard procedures.
The percentage of tumor cells in the fresh
tumor sample was est,lhlished by microscopic examination
of a frozen tissue section. DNA analyses
were performed as reported by Lekanne Deprez e(
a!. (199b). The following DNA markers for loci on
chromosome 22 were used: D2ZSIHI, D2ZS183
(Lekanne Deprez et aI., 1