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Nudeic Acids Research, Vol. 19, No.3
A new polymorphic probe on
chromosome 22: NB84 (0228183)
A.H.Lekanne Deprez', NAvan Biezen, P.Heutlnk 1 ,
K.R.G.S.8oejharat, Ada Klein 2 , A.H.M.Geurts van
Kessel 3 and E,C.Zwarthoff
Departments of Pathology, lClinicaJ Genetics and 2Cell
Biology and Genetics. Erasmus University and
JOepartment 01 Human Genetics, University of Nijmegen.
The Netherlands
A new polymorphic probe on
chromosome 22q: NB129
(0228193)
R.H.Lekanne Deprez', N.A.van Biezen, P.Heutinkl,
K.R.G.S.Boejharal, A.de Klein 2 , AH.M.Geurts van
Kessel 3 and E.C.Zwarthoff
Departments of Pathology, !Clinical Genetics and "Celt
Biology and Genetics, Erasmus University and
3Department of Human Genetics. University of Nijmegen,
The Netherlands
Source/Description: NB84 is a 1.0 kb HindJlI·EcoRI fragment
isolated from Iwo pooled chromosome 22 specific libraries (AT­
CC 1/ 57733 and ATCC 1/ 57714) and is subdoned into pUC9.
Polmwrphism: Pstl digestion of genomic DNA and hybridization
with the probe detects a two allele polymorphism: 4.6 kb (AI)
and 4.0 kb (Al). No constant bands were present.
Frequency: E5tirnated from 91 unrelated Caucasians.
AI: 0.19 A2: 0.81.
NOI PO(\'Inorphic For. Bgll, Bglll, DraI, Mspl and Taql.
Chmmosomalwcaliza!ion: Regionallocalizalion was established
by hybridization to a panel of somatic cell hybrids: PgMe-25Nu,
containing only human chromosome 22; PgMo-22 and
IC8-17ANu, respectively containing both products of the
Philadelphia translocation at 22ql 1; A3EW2-]8, containing the
t(lI:22) from Ewing's sarcoma (at 22q12) and 1122 AM27
containing a t((;22) at 22ql3 (I, 2). The probe was assigned to
chromosome 22 between 22pter and 22ql L
Mendelian Inheritance: Mendelian inheritance has been
demonstrated in extended pedigrees of Gilles de la TOllrette
syndrome families (n = ]80).
Probe Ami/abiliTY: Available for collaboration.
References: I) Goyns.M.H., YOllflg,8.D., Geurts van Kessel,A.,
de Klein,A., Gros~'eld,G., Bartram,C.R. and Bootsma,D. (1984)
Leukemia Res. 8, 547-553. 2) Geurts van Kessel,A., Turc­
Carel,C., de Klein.A .. Grosveld,G .. lenoir,G. and Bootsma,D.
(1985) Mol. Cdl Bioi. S, 427-429.
SourcelDescn'prion: NDI29 is a 0.6 kb HindllI-EcoRl fragment
isolated from twO pooled chromosome 22 specific libraries (AT­
CC # 5773] and ATCC # 57714) and is subdoned into pUC9.
Polymorphism: Pst! digestion of genomic DNA and hybridization
with the probe detects a two allele polymorphism: 1.6 kb (AI)
and 1.3 kb (Al). No constant bands were present.
Frequcncy: Estimated from 98 unrelated Caucasians
AI: 0.45 Al: 0.55.
Not Polymorphic For: Bgll, BglII, DraI, MspI and TaqI.
Olromosonlalwcaliza.tion: Regionallocaliz.ation was established
by hybridization to a panel of somatic cell hybrids: pgMe-25Nu,
containing only human chromosome 22; PgMo-22 and
ICB-17ANu, respectively containing both products of the
Philadelphia translocation at 22qI I; A3EW2 - ]B, containing the
t(lI;22) from Ewing's sarcoma (at 22q12) and 1122 AM27
containing a t(I;22) at 22qI3 (I, 2). The probe was assigned to
the long arm of chromosome 22 between 22qll and 22q12.
Mendelian Inhcritance: Mendelian inheritance has been
demonstrated in extended pedigrees of Gilles ,de la Tourette
syndrome families (n =:0 380).
Probe Amilability: Available for collaboration.
References: 1) aoyns,M.H., Young,B.D., Geurts van Kessel,A.,
de K1ein,A., Grosveld,G., Bartram,C.R. and Bootsma,D. (1984)
Leukemia Res. 8, 547-55]. 2) Geurts van Kessel,A., Turc­
Carel,C., de Klein,A., Grosveld,G" Lenoir,G. and Bootsma,D.
(1985) Mol. Cell Bioi. 5, 427-429.
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