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No. Days in Karyotypes and/or clonal rearrangements Ino. of cells]
culture
lnS1l' 6 44,X,del(X)(q13),trc(1 ;22; 19)(lqter->p12::22cen- > ql1:: 19cen-> qter),
inv(7)(p 14-ISq34-3S),der(lS),der(16)t(16;22)(q24;q?),i( 18p), and various
additional rearrangements in some cells [30]
176 17
177 10
180 5
183 6
46,XY [5JI
30-34,-22,incomplete (3)
46,XX [161
46,XX /16J
42-43,XY ,die(l; 19)(p 12;p 13)[23J, d,I(11 )(p 12)[2J,di,(11 ;21)(P II;p 12)/3),
tas( II ;21)(pter;pter)[51.-14(23),tas( 19 ;21)(qter;pter)[3] ,-21 (S) ,-22[23] (cp231
For abbreviations see legends table I.
Structural abnormalities of chromosome 22 were identified in 12 meningiomas. The
breakpoints observed after cytogenetical analysis were all in band 22qll (table 3). In 10
tumors we were able to map the breakpoints using a combined LOH and FISH study (figures
1,2). Five tumors (9, 22, 39, 41 and 140) showed retention of proximal chromosome 22
markers and loss of distal markers with breakpail;ts scattered between D22S 181 and 17.1 3'.
Tumor 121, a meningioma in an NF2 patient, revealed an interstitial deletion with the
proximal breakpoint between D22S 181 and D22S 183 and the distal breakpoint between
D22S29 and D22S45. In the tumors 51 and 144 loss of proximal chromosome 22 markers
and retention of distal markers were observed. In these latter meningiomas chromosome 22
could either be involved in an interstitial deletion or a translocation resulting in a deletion
of the proximal part of this chromosome. Two tumors (32, 82) showed structural aberrations
of both chromosome 22 copies (table 3). In meningioma 32 one copy of chromosome 22 was
involved in a reciprocal translocation t(4;22) from which the 22q- and the 4p+ derivative
chromosomes were the products and the other copy (22q +) was involved in a translocation
with chromosome 1 resulting in der(22)t(l ;22) as previously reported (fig.2; Lekanne Deprez
et aI., 1991a). FISH and RFLP analysis on hybrid cell lines segregating the reciprocal
translocation products and on cultured cells derived directly from meningioma 32 enabled us
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