XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta

Lectures
FUNCTION AND PROPERTIES OF HEarT AND KIDNEY MITOCHONDRIA
(MIT) IN SPONTANEOUSLY HYPERTENSIVE (HYP) raTS: INFLUENCE OF
CAPTOPRIL AND NIFEDIPINE
Attila Ziegelhöffer 1 , Jana Mujkošová 1 , Oľga Uličná 2 , Iveta Waczulíková 3 , Miroslav ferko 1 ,
Norbert Vrbjar 1 , Štefan Polák 4 , Tanya Ravingerová 1 and Adriana Adameová 5
1
Inst Heart Res SAS, 2 Pharmacobioch Lab III rd Med Clin MF UK, 3 Dept Biomed Physics
FMFI UK, 4 Inst Histol Embryol MF UK, 5Inst Pharmacol Toxicol, PaF UK, Bratislava
There are only scarce data available about HYP-induced changes in function and properties
of heart MIT and multi-organ studies also involving the effect of anti-HYP treatment
with captopril (CAP) or captopril + nifedipine (CAPNI) are missing completely. In present
study we complete this lack by estimating O 2
consumption and oxidative phosphorylation
(Oxygraph Gilson), DNP stimulated Mg-ATPase activity and membrane fluidity
(fluorescence probe DPH) in MIT isolated from heart and kidney of 16 weeks old HYP
rats treated for the next 4 weeks with CAP (80 mg.kg -1 daily) or CAPNI (80 CAP+10 NI
mg.kg -1 daily). HYP induced a moderate elevation of MIT ATP production in response to
HYP-induced increase in energy demands (ED) of the heart. By removing the HYP, both
treatments also decreased the ED and normalized the MIT ATP production. In kidney MIT
HYP decreased the ATP production by depressing the values of OPR. Both treatments,
particularly that with CAPNI even deepened this damage.
Acknowledgements: Grants: VEGA 1/0620/10; 2/0173/08..
XXII. Biochemistry Congress, Martin
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