XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta

Posters
46.
DifferENCES IN INTEraCTION of LECTINS SPECIfICaLLY rECOGNIZING
SIaLIC aCID rESIDUES WITH SUrfaCE of P-GP NEGaTIve or POSITIve
L1210 CELLS
Tatiana Kurucová, Helena Kavcová, Kristína Rogozanová, Lucia Messingerova,
Danica Mislovičová 1 , Albert Breier and Zdena Sulová
Institute of Molecular Physiology and Genetics SAS, Bratislava, Slovakia
1
Institute of Chemistry SAS, Bratislava, Slovakia
Multidrug resistance (MDR) of mouse leukemic cell line L1210/VCR (R) obtained by
adaptation of L1210 cells (S) to vincristine (VCR) is accompanied by overexpression of
P-glycoprotein (P-gp). Selection with VCR that confers overexpression of P-gp is also inducing
various kinds of metabolic alteration. We described recently several differences
in cell surface saccharides between R and S cells.
Aim of this study was to resolve in exist differences in interaction of Triticum vulgaris
lectin (WGA), Maackia amurensis lectin (MAA), Sambucus nigra lectin (SNA) with cell
surface of S, R cells and L1210 cells that express P-gp due to transfection of cells with
plasmid containing human gene encoding this protein (T cells). While MAA was found to
be nontoxic for S, R and T cells and SNA is exerting small cell damage effect, WGA induced
concentration depended cytotoxic effect. Agglutination of cells by this lectin is more massive
to cells expressed P-gp. WGA lectin interact more potently with P-gp positive cells
R and T as with P-gp negative S cells. Spectrum of cell membrane glycoprotein targets
of lectins were assessed by lectin blot methods. Our data indicated that application of
lectins enable to study the differences cell surface saccharides.
Acknowledgements: This work was supported by: APVV-0084-07, VVCE-0064-07, VEGA-
2/0123/10, VEGA-2/0155/09.
XXII. Biochemistry Congress, Martin
165