XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta

Posters
96.
AntioxidaNT capaCITY of SELECTED anaLOGS of NUCLEIC aCID
COMPONENTS: COMParISON of CELL-frEE aND CELL-baSED aSSaYS
Eliška Procházková, Petr Jansa, Lucie Čechová, Ivan Votruba and
Helena Mertlíková-Kaiserová
Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech
Republic, Gilead Sciences & IOCB Research Center, 166 10 Prague, Czech Republic
Overproduction of reactive oxygen species (ROS) is a phenomenon accompanying a number
of human diseases as well as drug toxicity. Pharmacological potential of the analogs
of nucleic acid components is of considerable interest and so is their ability to interfere
with ROS production. In this study we used a series of poly-substituted pyrimidines
and purines in order to explore their antioxidant capacity. Their ability of direct radical
scavenging was first determined with use of the trolox equivalent antioxidant capacity
(TEAC) assay. Consequently, the effects of the tested compounds on Fe 2+ /ascorbateinduced
lipid peroxidation in rat liver microsomes were studied. Finally, ROS cellular
levels were traced in compound-pretreated HepG2 cells exposed to oxidative stress by
means of a redox-sensitive fluorescein-based probe CM-H 2
DCFDA. Although remarkable
antioxidant capacity of several compounds was detected in individual assays, we
observed that the results largely depend on the method used and the data therefore
have to be interpeted with caution. We suggest that these variations could be due to
different physico-chemical properties of the compounds.
Acknowledgement: This work was supported by the Research project of the IOCB
OZ40550506 and the Project #1M0508 by the Ministry of Education, Youth and Sports
of the Czech Republic.
220 XXII. Biochemistry Congress, Martin