XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta

Posters
52.
ISOFORMS OF AMP-ACTIvaTED PROTEIN KINASE SUBUNITS IN
LYMPHOCYTES AND OBESITY
Boris Lakatoš, Lucia Bialešová and Eva Harnišová
Institute of Biochemistry, Nutrition and Health Protection, Department of Biochemistry
and Microbiology, Faculty of Chemical and Food Technology, Slovak University of
Technology, Radlinského 9, 812 37 - Bratislava, Slovakia
Obesity becomes to be a real problem in developed countries and it seems that its
prevalence increases. One of consequences of obesity is the increasing incidence of
metabolic syndrome - a state of metabolic dysregulation characterized besides obesity
also with resistance to insulin, changes in blood lipids level, early atherosclerosis, changes
in blood tension, which can result to the appearance of civilization diseases. Although
the obesity could be caused by different factors, studies in last decades pointed out on
fundamental mechanisms leading to the defects in lipid metabolism, which are regulating
energy fluxes in animal metabolism. It is known that 5´-AMP-activated protein kinase
(AMPK) a sensor of metabolic status plays the key regulatory role. Structurally is AMPK
heterotrimeric complex consisting of catalytic α subunit and regulatory β and γ subunits
existing in several isoforms (α 1
, α 2
, β 1
, β 2
, γ 1
, γ 2
, γ 3
). AMPK is widely distributed in all body
tissues. In this work we followed expression of AMPK subunits isoforms in human lymphocytes
isolated from blood of undergraduate students of FCHFT SUT. Simultaneously
we measured basic biochemical parameters of lipid, glucose and energy metabolism.
The obtained values were used to create correlation dependence with body mass index
(BMI) and physical activity.
We found expression of genes for α 2
, β 2
and γ 3
subunits in all blood samples regardless of
gender, BMI or physical activity of probands. Sequential analysis of obtained PCR products
for α 2
subunit of AMPK did not show any polymorphisms. Biochemical parameters
showed rather weak, if any, correlation with BMI.
Acknowledgements: This work was supported by the grant VEGA, nr. 1/0589/08.
172 XXII. Biochemistry Congress, Martin