XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta

Posters
77.
Rep 34
prOTEIN ENCODE BY PLaSMID pGP2 frOM aCetobaCTer
Peter Grones, Zuzana Odnogová and Jozef Grones
Comenium University, Department of Molecular Biology, Bratislava
The Acetobacter estunensis Rep 34
protein participates in the replication of bacterial small
cryptic plasmid pGP2. The Rep 34
protein (213 aa, 23.65 kDa) from Acetobacter plasmid
pGP2, was cloned to the expression vector, that ensure fusion with a His-tag sequence
(Rep 34
His-tagged), over-expressed in Escherichia coli and purified by metal-affinity
chromatography to yield a highly purified and active protein. On this purified protein
number different activities and motifs were detected. DNA band-shift assays showed that
the Rep 34
His-tagged protein bound to the regulation region for replication on the linear
double-stranded DNA. In the protein was determined phosphatase activity, ATPase activity
and protein is possible to unwind double strand DNA. After fusion with GFP protein the
accruement of protein expression with confocal microscopy was proven.
200 XXII. Biochemistry Congress, Martin