XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta

Posters
21.
phiGENOME – a WEB-baSED GENOME brOWSEr INTENDED FOR
DISPLAY OF PHaGE GENOMES
Matej Stano and Ľuboš Kľučár
Institute of Molecular Biology, SAS Bratislava
Graphical representations of genomes convey quick insight into organization of coding
sequences and other functional regions within genomes. Applications intended for generation
of graphical representations of genomes are genome browsers.
We present phiGENOME, a web-based genome browser providing highly intuitive and
interactive maps of phage genomes stored in phiSITE – database of gene regulation in
bacteriopages. It provides dynamic genome maps built on the Adobe Flash platform as
well as an accessory semi-graphics based on precisely formatted HTML tags. phiGENOME
is a integral part of phiSITE web interface, therefore it was optimized for visualization of
phage genomes with emphasized display of cis-regulatory elements. Graphical content
is generated dynamically; required annotation data are retrieved promptly from the
MySQL back-end. The data transfer from the database to the genome browser is supplied
via XML files.
Genome maps acquired from phiGENOME allow graphically subtle and lucid inspection
of phage genomes. Individual genome features are displayed as coloured boxes; genes:
blue, promoters: red, operators: green, terminators: yellow. All actions in this browser
can be comfortably performed with mouse. Mouse drag enables continuous sliding along
genome; mouse scroll zooms in (up to the level of nucleotide sequence) and out; mouse
over a feature displays tool-tip with feature details; mouse click highlights selected feature
and opens options menu for further actions. phiGENOME is an innovatory application
according to the mode and purpose how the Flash technology was employed. It is freely
accessible at phiSITE web interface: http://www.phisite.org.
XXII. Biochemistry Congress, Martin
137