XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta

Posters
2.
IS IT POSSIBLE TO IMPROVE DEMYELINATION DISEASES MONITORING BY
DETERMINATION OF SOME ENZYME ACTIVITIES CHaraCTERISTIC FOR
THE CENTraL NErvOUS SYSTEM?
Monika Ďurfinová 1 , Marta Brechtlová 1 , Ľubica Procházková 2 , Peter Kukumberg 2 ,
Ľubomír Kuračka 1 and Branislav Líška 1
1
Department of Chemistry, Biochemistry and Clinical Biochemistry, LF UK Bratislava,
2
2 nd Department of Neurology, LF UK Bratislava
Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system
(CNS). It is the most common neurological disease characterized by recurrent relapses
and/or progression that are attributable to multifocal inflammation, demyelination and
axonal pathology within the CNS. MS lesions are typically located in the periventricular
white matter of the brain as well as in superficial areas of the spinal cord. Since MS
lesions are not routinely biopsied, the cerebrospinal fluid (CSF) is used for measurements
of various soluble markers. We have started determination of enzyme activities
in the CSF of MS patients, which have some relationship to the function of the CNS
tissue – phosphodiesterase 3 , ,5 , -cAMP and K + -paranitrophenyl-phosphatase. These
enzyme activities were at first monitored in model experiments in vitro. We suppose
that K + -paranitrophenylphosphatase might have a connection with the nerve impulse
transmission. Interestingly, the part of phosphodiesterase 3 , ,5 , -cAMP activity, which is
calcium-calmodulin dependent, was inhibited by the conditions of activated oxidative
stress. However, several questions still remain to be elucidated, e.g.: if this CNS inflamatory
damage could infuence releasing of these enzymes into CSF and if these enzymes
can reflect the intensity of the patological process.
116 XXII. Biochemistry Congress, Martin