XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta

Posters
9.
EffECT of METHYL jaSMONaTE ON THE prODUCTION of
saNGUINarINE aND ITS prECUrSOrs IN OPIUM POPPY SUSPENSION
CULTUrES
Andrea Balažová 1 , Víťazoslava Blanáriková 1 , Jindra Valentová 2 ,
František Bilka 1 and Ivana Holková 1
1
Department of cellular and molecular biology of drugs, FaF UK Bratislava
2
Department of chemical theory of drugs, FaF UK Bratislava
Suspension cultures of opium poppy are not able to produce morphinan alkaloids but
synthesize and store sanguinarine within a single cells. The morphinans and sanguinarine
share common biosynthetical pathway, leading to (S)-reticuline as a crutial intermediate.
The biosynthesis of these alkaloids begins with the conversion of tyrosine to both
dopamine and 4-hydroxyphenylacetaldehyde. The suspension cultures of opium poppy
enhenced their sanguinarine production after treating with three different concetrationes
of methyl jasmonate (10, 100, 1000 μmol/l). The presence of elicitor in nutrient medium
of suspension cultures also led to the changes in levels of sanguinarine precursors (DOPA,
dopamine, tyrosine and tyramine). The suspension cultures were exposed to elicitror
treatment for 2, 4, 6, 8, 10, 24 and 48 h. The maximum content of sanguinarine was
detected after 8 h exposure to elicitor at concetration 100 μmol/l (5.8-fold increase over
the control). The suspension cultures produced the highest amount of DOPA between
2-6 h after elicitation with all three concetrationes of methyl jasmonate. The level of
dopamine increased gradually in elicited cultures. The maximal content of dopamine was
detected after 8 h exposure to elicitor at concentration 10 μmol/l (3.24-fold increase).
The level of tyrosine started to increase later than the levels of DOPA and dopamine. The
accumulation of tyrosine was positively affected mainly by methyl jamonate at concetrationes
10 and 100 μmol/l. The levels of tyramine have not been changed significantly
in elicited suspension cultures.
124 XXII. Biochemistry Congress, Martin