XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta

Lectures
CHANGES AND ROLE OF ADRENOCEPTORS IN PC12 CELLS afTER
PHENYLEPHRINE ADMINISTraTION AND APOPTOSIS INDUCTION
Ľubomíra Lenčešová 1,2 , Marta Sírová 1 , Lucia Csáderová 2 , Marcela Lauková 3 ,
Zdena Sulová 1 , Richard Kvetňanský 3 and Oľga Križanová 1
1
Institute of Molecular Physiology and Genetics, Center of Excellence
for Cardiovascular Research, Slovak Academy of Sciences, Bratislava, Slovak Republic,
2
Molecular Medicine Center, Slovak Academy of Sciences, Bratislava, Slovak Republic,
3
Institute of Experimental Endocrinology, Slovak Academy of Sciences,
Bratislava, Slovak Republic
Adrenergic regulation might modulate the effect of apoptosis. Therefore we studied,
whether a1-adrenergic receptor’s agonist phenylephrine (PE) can affect or induce apoptosis
in rat pheochromocytoma (PC12) cells. We have shown that PE treatment did not
increase level of the apoptosis, or level of the caspase 3 mRNA. When apoptosis was
induced in the presence of PE, caspase 3 mRNA was significantly increased, while the
percentage of apoptotic cells remained unchanged compared to apoptotic group without
PE. During this process, a1D-, b2- and b3-adrenergic receptors (ARs) were upregulated.
Since all these three types of ARs are differently localized in the cell, we assume that
mutual communication of all three ARs is crucial to participate in this signaling and during
development of apoptosis, some of these systems might translocate. Another inportant
system in handling noradrenaline during apoptosis might be noradrenaline transporter
(NET), since it was downregulated in apoptotic cells treated with PE, compared to untreated
apoptotic cells. However, precise mechanism of mutual communication among
all these systems remains to be elucidated.
This work was supported with scientific grants APVV 51/0397 and VEGA 2/0049/10.
XXII. Biochemistry Congress, Martin
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