XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta

Lectures
BIOMarKErs of LYMPH NODE METaSTaSIS IN LOW-graDE breaST
caNCEr: aN INTEGraTED, prOTEOMICS-baSED aPProaCH
Pavel Bouchal 1,2 , Monika Mudrochová 1,2 , Eva Budinská 3 , Zbyněk Bortlíček 3 ,
Iva Struhárová 1,2 , Lenka Hernychová 4 , Theodoros Roumeliotis 5 , Spiros D. Garbis 5 ,
Roman Hrstka 1 , Petr Müller 1 , Rudolf Nenutil 1 and Bořivoj Vojtěšek 1
1
Masaryk Memorial Cancer Institute, Brno; 2 Masaryk University, Faculty of Science, Brno;
3
Masaryk University, Institute of Biostatistics and Analyses, Brno;
4
University of Defence, Faculty of Military Health Sciences, Hradec Králové,
5
Academy of Athens, Greece
Our effort has been focused on biomarker discovery in the set of 96 breast cancer tumors
divided into groups according to grade and presence or absence of lymph node metastases.
Three proteomics approaches were involved in complex protein analysis of tissue
lysates: (i) SELDI-TOF MS enabled us to quantify 130 intact protein peaks. One protein
peak correlating with lymph node metastases was detected and identified. (ii) Selected
set of tumors was analyzed using iTRAQ-2DLC-MS/MS approach. This approach provides
a simultaneous identification and quantitation of more than 600 proteins. Differentially
expressed proteins belong to several functional groups which will be described in the
presentation. (iii) Set of selected tumor lysates was analyzed also using 2-D SDS-PAGE.
Additionally, the expression variability of 26 selected gene products was evaluated using
qRT-PCR.
Generally, our results indicate the differential expression of (i) cytoskeletal proteins, (ii)
anterior gradient protein family members and (iii) proteins involved in heme biosynthesis
between the two studied groups.
Acknowledgments: This work was supported by Czech Science Foundation (Project No.
304/10/0868), by Czech Ministry of Health (Project No. MZMOU2005) and by Czech
Ministry of Education (MSM0021622413).
50 XXII. Biochemistry Congress, Martin