XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta
XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta
XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta
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Posters<br />
61.<br />
IS RESPIraTORY PATHWAY ACTING THROUGH NO-sGC?<br />
Ľudmila Capková, Alexandra Dávidová, Andrea Kucháriková and Nadežda Lukáčová<br />
Institute of Neurobiology, Slovak academy of Sciences, Košice<br />
Most effects of the nitric oxide (NO) are mediated by stimulation of soluble guanylyl<br />
cyclase (sGC) and subsequent increase in cyclic guanosine monophophate (cGMP) formation.<br />
NO/sGC/cGMP signalization was identified in neuronal pathways of the brain,<br />
but little is known about this signaling pathway in the spinal cord. The aim of our study<br />
was to find out, whether bulbospinal respiratory pathway is acting through NO-sGC<br />
signalization. This pathway begins in medulla and project to the phrenic motoneurons<br />
controlling diaphragm activity. The distribution of the neuronal nitric oxide synthase<br />
(nNOS), β1 subunit of soluble guanylyl cyclase (β1sGC) and synaptophysin (SYN) was<br />
explored in control animals and after C2-C3 spinal cord hemisection in upper part of the<br />
respiratory pathway. Retrograde tracer Fluorogold (FG) was used for identification of<br />
respiratory neurons in medulla. Two days after injection of FG into the phrenic nucleus<br />
(PN) at C4 level revealed amount of FG fluorescent neurons in the ventral respiratory<br />
group (VRG) mostly on contralateral side. We showed intense punctate nNOS and SYNpositive<br />
terminals of respiratory neurons in neuropile of PN in control animals and strong<br />
depletion of these terminals on contralateral side and almost entire depletion of nNOS<br />
and SYN-positive terminals on ipsilateral side of lesioned animals. Eight days after the<br />
hemisection we have revealed lightly ß1sGC fluorescent motoneurons of PN and around<br />
them a few nNOS fluorescent boutons on contralateral side. Almost no sign of ß1sGC<br />
immunopositivity could be seen ipsilaterally to the hemisection. These results together<br />
suggest that bulbospinal respiratory pathway is acting through NO-sGC.<br />
Ackowledgement: The experimental work was supported by VEGA Grant 2/0015/08.<br />
<strong>XXII</strong>. Biochemistry Congress, Martin<br />
183
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