XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta

Posters
66.
EffECT OF OMEGA-3 PUfa ON LIPID PROFILE AND OXIDATIVE STRESS IN
HYPERCHOLESTEROLEMIC CHILDREN
Iveta Ondrejovičová 1 , Jana Muchová 1 , Zuzana Paduchová 1 ,
Zuzana Nagyová 2 and Zdeňka Ďuračková 1
1
Institute of medical chemistry, biochemistry and clinical biochemistry,
Medical Faculty, Comenius University, Bratislava, Slovak Republic
2
Juvenalia, s.r.o., Pediatric center, Dunajská Streda, Slovak Republic
Hypercholesterolemia is defined as an impairment of lipid metabolism. It is characterized
by an increased level of cholesterol above 6.2 mmol/L in adults and above 4.2 mmol/L in
children. Several studies have proved that every fourth child in Slovakia older than 11 – 12
years has increased cholesterol levels. The aim of our clinical study was to monitor changes
in lipid profile in 35 children with a mild hypercholesterolemia (average age 16 ± 3.34 years),
after daily supplementation with a nutritional product containing omega-3 polyunsaturated
fatty acids (PUFA) (1000 mg EPA/DHA) and phytosterol esters (1300 mg) during 16
weeks period. Oxidative stress also participates in pathogenesis of hypercholesterolemia
and therefore we have monitored the effect of this product on markers of oxidative damage
to lipids (lipid hydroperoxides, 8-isoprostanes) and the total antioxidative status. We
collected samples before and after 8 and 16 weeks of administration of the supplement.
Serum/plasma was prepared by a standard procedure and was stored at -20 ºC. The total
cholesterol (TCH), LDL-cholesterol (LDL), HDL-cholesterol (HDL), triacylglycerols (TAG),
C-reactive protein, lipoprotein-A, glucose, uric acid, as well as parameters of oxidative stress
were analyzed in serum/plasma samples. After 16 weeks of supplement administration we
have found out that levels of TCH (5.24–4.86 mmol/L, p