XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta

Posters
99.
EffECT OF RENAL TraNSPLANTATION ON OXIDATIVE STRESS-RELATED
BIOMarKERS
Adéla Zdařilová 1 , Alena Rajnochová Svobodová 1 , Jana Zapletalová 2 , Pavel Štrebl 3 ,
Josef Zadražil 3 and Jitka Vostálová 1
1
Department of Medical Chemistry and Biochemistry, 2 Department of Medical
Biophysics, Faculty of Medicine and Dentistry, Palacký University, Hněvotínská 3,
775 15 Olomouc; 3 Department of Internal Medicine III University Hospital,
I. P. Pavlova 6, 775 20 Olomouc, Czech Republic
Patients after kidney transplantation (KT) have a high prevalence of oxidative stress (OS)
that is associated with the excess of cardiovascular complications observed in this population.
Determination of OS-related parameters together with specific kidney function
markers is used to monitor graft function.
Time-dependent changes in OS-related markers in patients (N = 39; male = 23; female
= 16; mean age = 57 ± 10) before (day 0) and after KT (day 1, 7, 30, 90 and 180) were
evaluated. Total antioxidant capacity (TAC), levels of advanced oxidation protein products
(AOPP), lipid peroxidation as thiobarbituric acid-reactive substances (TBARS) and
reduced glutathione (GSH), activities of glutathione peroxidase (GPX), catalase (CAT) and
superoxide dismutase (SOD) and kidney function markers were measured.
AOPP, TAC and TBARS were significantly decreased whereas GSH was significantly increased
after KT. Antioxidant enzyme activities did not changed after KT during monitored period.
Of specific kidney function markers glomerular filtration was significantly increased and
creatinine level was significantly decreased after KT.
Acknowledgements: This work was supported by grants MSM 6198959216 and MZ CR
(NS/9964-4).
XXII. Biochemistry Congress, Martin
223