XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta

Posters
49.
DIMETHYL-OXALYLGYCINE MODULATES GENE EXPRESION AND PROTEIN
LEVELS OF THE SODIUM CALCIUM EXCHANGER IN HEK 293 CELL LINE
Cagala M. 1 , Lencesova L. 1,2 , Hudecova S. 1 , Csaderova L. 2 , Sirova M. 1 ,
Cholujova D. 3 , Kopacek J. 4 , Pastorekova S. 2,4 and Krizanova O. 1,3
1
IMPG, Center of Excellence for Cardiovascular Research, SAS, Vlarska 5,
833 34 Bratislava, Slovak Republic 2 MMC, SAS, Vlarska 3-7, 831 01 Bratislava,
Slovak Republic 3 IEO, SAS, Vlarska 7, 833 91, Bratislava, Slovak Republic 4 IV,
Center of Excellence for Cardiovascular Research, SAS, Dubravska cesta 9,
845 05 Bratislava, Slovak Republic
Up to now a little is known about the effect of hypoxia on the NCX1 expression and
function. Therefore we studied, how dimethyl-oxalylglycine (DMOG), an activator and
stabilizator of the HIF-1α could affect expression of the NCX1 in HEK 293 cell line. We also
tried to determine, whether this activation can result in the development of apoptosis in
HEK 293 cells. We have found that DMOG treatment for 3 hours significantly increased
gene expression and also protein levels of the NCX1. This increase was accompanied
with the decrease in intracellular pH. Wash-out of DMOG did not result in decrease of
NCX1 mRNA and protein to original, control levels, although pH returned to control
values. In the promoter region of the NCX1 we did not find consensus sequence for
HRE, but we have found consensus NF-kB sequence in this region of the NCX1. Using
luciferase reporter assay we observed increase in NCX1 promoter activity after DMOG
treatment, which suggests that NF-kB is involved in DMOG induced upregulation of
the NCX1. Moreover, we also showed that this upregulation might be involved, at least
partially, in the induction of the process of apoptosis. Nevertheless, further experiments
are needed to clarify this issue.
Ackowledgement: This work was supported by grants APVV 51-0397-07, VEGA 2/0082/10
and TRANSMED.
XXII. Biochemistry Congress, Martin
169