XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta

Posters
117.
ELLIPTICINE CYTOTOXICITY TO HUMan THYrOID caNCEr CELL LINES
Jitka Poljaková 1 , Tomáš Eckschlager 2 , Eva Frei 1 and Marie Stiborová 1
1
Department of Biochemistry, Faculty of Science, Charles University in Prague,
2
Department of Pediatric Hematology and Oncology,
2 nd Medical School, Charles University in Prague
Ellipticines are plant alkaloids with an antineoplastic activity. The mode of action is based
mainly on DNA intercalation, inhibition of topoisomerase II and formation of covalent
DNA adducts mediated by cytochromes P450 (CYP) and peroxidases. Such ellipticine-
DNA-adducts are formed in vitro, in human breast adenocarcinoma MCF-7, leukemia
HL-60, CCRF-CEM, neuroblastoma IMR-32, UKF-NB-3, UKF-NB-4 cell lines and in vivo in
rats exposed to ellipticine. Here, the cytotoxicity of ellipticine to human thyroid cancer
cell lines BHT-101, B-CPAP and 8505-C was investigated. Furthermore, the effect of hypoxic
conditions on the ellipticine toxicity to these cells was also examined. The toxicity
of ellipticine to thyroid cancer cells cultivated under the standard conditions was higher
than that to the cell lines grown under hypoxia (1% oxygen). Another target of this work
was to evaluate the effect of ellipticine on expression of enzymes activating ellipticine,
namely on CYP1A1, 1B1, 3A4, cytochrome b 5
and peroxidases COX-1 and TPO.
Acknowledgement: Supported by GACR (P301/10/0356) and Czech Ministry of Education
(MSM0021620813).
242 XXII. Biochemistry Congress, Martin