XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta

Posters
111.
ASSOCIATION POLYMORPHISMS of GST, hOGG1 aND XRCC1 GENES
WITH LUNG aDENOCarCINOMa
Tatiana Matáková 1 , Erika Halašová 2 , Lucia Letková 2
Anton Dzian 3 and Dušan Dobrota 1
1
Institute of Medical Biochemistry, JMF CU in Martin; 2 Institute of Medical Biology,
JMF CU in Martin; 3 Clinic of Surgery, JMF CU and MFH in Martin; Slovak Republic
A case-control study with 130 lung cancer cases and 290 controls was conducted. DNA
was extracted from peripheral blood leukocytes, and the polymorphisms of GSTM1,
GSTT1, GSTP1, XRCC1 and hOGG1 enzymes were determined by PCR-based methods.
In this study we determined the genotype distribution of all these genes, and their combinations.
Association between specific genotypes and the development of lung cancer
were examined using logistic regression analysis to calculate odds ratios (OR) and 95% CI.
The GSTT1 null genotype (OR=2.2; p=0.004) was associated with an elevated risk. The
statistically significant correlation was found also for the combined genotypes of GSTT1
null and GSTM1 positive (OR=2.2; p=0.04) and GSTT1 null and GSTP1 Ile/Val or Val/Val
(OR = 2.9; p = 0.009). For hOGG1 and XRCC1 were no difference in the frequency of
genotypes between matched cases and controls. The statistically significant correlation
was found also for the combined genotypes of GSTT1 null and GSTM1 positive (OR=2.2;
p=0.04) and GSTT1 null and GSTP1 Ile/Val or Val/Val (OR=2.9; p=0.009).
The statistically significant correlation was found for the combined genotypes of GSTT1
null and hOGG1 Ser/Ser (OR=2.2; p=0.02) and GSTT1 null and XRCC1 Arg/Arg (OR=2.7;
p=0.03).
The genotype of selected enzymes affects the risk for lung adenocarcinoma.
Acknowledgments: This work was supported by Ministry of Health of the Slovak republic
under the project No. 2007/48-UK-13 and project “Center of translational medicine”
cofinanced from EC sources and European Regional Development Fund.
236 XXII. Biochemistry Congress, Martin