XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta

Lectures
INHIBITION OF INSULIN AMYLOID AGGREGATION WITH ALBUMIN
FUNCTIONALIZED MAGNETIC FLUID
Andrea Antošová 1 , Katarína Šipošová 2 , Martina Koneracká 1 , Vlasta Závišová 1 ,
Peter Kopčanský 1 and Zuzana Gažová 1
1
Department of Biophysics, Department of Magnetism, Institute of Experimental
Physics SAS, Kosice, Slovakia, 2 Department of Biochemistry, Faculty of Science,
P. J. Safarik University, Kosice, Slovakia
Amyloid-related diseases, such as Alzheimer’s disease or diabetes type II, are associated
with self assembly of protein into amyloid aggregates. Recently, there are only few reports
dealing with the effect of nanomaterials on the amyloid aggregation. We investigated
effect of magnetic fluid consists of Fe 3
O 4
nanoparticles sterically stabilized by sodium
oleate with adsorbed BSA (MF-BSA) on amyloid aggregation of insulin. The ability of MF-
BSA to inhibit formation of insulin amyloid aggregates (Iagg) in vitro was studied by ThT
assay and TEM. We have found that MF-BSA is able to prevent formation of aggregates,
the extent of amyloid formation depends on MF-BSA concentration with extensive 70%
inhibiting activity for ratio Iagg:MF-BSA = 1:7. The obtained results indicate that presence
of MF-BSA led to the inhibition of insulin amyloid aggregation. Our findings make
MF-BSA of potential interest as therapeutic agents against amyloid-related diseases.
Ackowledgement: This work was supported within the projects Nos. 26220220005,
26220120033 and 26220120021 in frame of SF EU, Centre of Excellence of SAS Nanofluid,
VEGA 0079, 0056, 0077 and VVGS PF 13/2010/ Ch.
XXII. Biochemistry Congress, Martin
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