XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta
XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta
XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta
You also want an ePaper? Increase the reach of your titles
YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.
Lectures<br />
MAGNETIC RESONANCE SPECTROSCOPY IN DIAGNOSTIC PROTOCOL OF<br />
THE BraIN DISEASES<br />
Dušan Dobrota and Michal Bittšanský<br />
Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava,<br />
Slovak Republic<br />
Magnetic resonance spectroscopy (MRS allowed study of some biochemical changes,<br />
and metabolic pathway in vitro and in vivo. MRS is a physical technique that has been<br />
used as an analytical method, predominantly by chemists to describe the structure of<br />
molecules in a specific solution. In biological application the method allowed to study<br />
low molecular compounds containing atoms that have magnetic properties (e.g. 1 H, 31 P,<br />
13<br />
C, 19 F etc..Proton magnetic resonance spectroscopy ( 1 H MRS) can measure levels of<br />
cerebral metabolites with the low molecular weight such as N-acetylaspartate (NAA),<br />
choline (Cho), creatine (Cre), lactate (Lac) and some others. 1 H MRS application in vivo in<br />
the diagnostic protocol some brain diseases (brain tumors, epilepsy, neurodegenerative<br />
diseases and schizophrenia) was the study’s focus. In vivo magnetic resonance spectra<br />
were obtained from the different parts of the brain using clinical scanner Siemens<br />
Symphony (1,5T) and standard protocols. 1 H magnetic resonance spectroscopy (MRS)<br />
provides valuable information about the changes in the concentration of above mentioned<br />
metabolites and their ratios, which are typical for each brain diseases. The great<br />
advantage of MRS in vivo study is that we are allowed study of these biochemical events<br />
in real time without any disturbance of the tissue. We may conclude, that in vitro 1 H MRS<br />
provides good information about biochemical differences in various type of human brain<br />
pathologies and could be used in the standard diagnostic protocol.<br />
Acknowledgement: This work was supported by the Ministry of Education Grant 048-<br />
010UK-8/2008 and by project “CENTER OF TRANSLATIONAL MEDICINE” co-financed from<br />
EU sources and European Regional Development Fund.<br />
54 <strong>XXII</strong>. Biochemistry Congress, Martin
Change language