XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta

Posters
10.
COMParISON OF SANGUINarINE PRODUCTION OF PAPavEraCEAE
faMILY PLANTS IN VITro CULTURES
František Bilka, Andrea Balažová, Andrea Bilková, Víťazoslava Blanáriková,
Ivana Holková and Marián Vanko
Department of cellular and molecular biology of drugs, Faculty of Pharmacy,
Comenius University in Bratislava
Intact plants of Papaveraceae family are producers of the whole range of benzylisoquinoline
alkaloids, which are used in pharmaceutical industry. In vitro cultures derived from
plants of Papaveraceae do not have the ability to produce so broad spectrum of alkaloids
– active is only biosynthetic pathway leading to sanguinarine. We derived in vitro cultures
of Papaver somniferum, Eschscholtzia californica, Chelidonium majus and Macleaya
cordata. Their sanguinarine production abilities were tested and compared. Also the
effect of elicitation on the sanguinarine production was studied. The lowest amounts of
sanguinarine from all cultures tested were accumulated in suspension cultures of opium
poppy (0.45 – 0.55 mg in 1 g of fresh weight). Eschscholtzia calicornica, Chelidonium majus
and Macleaya cordata cultures produced similar amounts of sanguinarine (18.0 – 22.7 mg;
20.5 – 26.3 mg; 15.4 – 20.3 mg in 1 g of fresh weight, resp.). In elicitation studies we used
biotic (Botrytis cinerea hydrolysate) and abiotic (CuSO 4
, methyljasmonate) stressors. In
all cultures treated the increase in sanguinarine accumulation was observed. Among the
elicitors used the most effective was B. cinerea hydrolysate. From all cultures tested the
most intensive response was observed in opium poppy cultures (increse to almost 9 mg
of sanguinarine in 1 g of fresh weight), although the amount of sanguinarine in elicited
poppy cultures was lower than in non-elicited cultures of the other cultures.
XXII. Biochemistry Congress, Martin
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