XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta

Posters
25.
EXPrESSION of traNSCrIPTION faCTOrs aND MICrOrNaS
IN TGF-SS1–INDUCED EMT of BENIGN prOSTaTE EPITHELIal CELLS
E. Lincová/Slabáková 1,2 , Zuzana Pernicová 1,2 , Eva Slavíčková 1,2 ,
Alois Kozubík 1,2 and Karel Souček 1
1
Dept. of Cytokinetics, Institute of Biophysics AS CR, Brno, Czech Republic.
2
Dept of Exp. Biology, Masaryk University, Faculty of Science, Brno, CZ.
Epithelial-mesenchymal transition (EMT), in which epithelial cells become motile mesenchymal
cells, is viewed as an essential early step facilitating dissemination of tumour
cells. MicroRNAs (miRNAs) are small non-protein-coding regulators of gene expression
that play an important role in tumorigenesis and, depending on their targets, can
function as tumour suppressors or oncogenes. Members of the miR-200 family, which
regulate expression of ZEB transcription factors, have been found downregulated during
EMT, suggesting an important role in inhibition of EMT. The aim of this study is to
analyze the kinetics of expression of selected transcription factors and miRNAs in the
EMT of prostate cells in order to identify key molecules responsible for tumorigenicity
and invasivity of prostate cancer.
In our experimental system, we induced EMT by TGF-ß1 treatment in BPH-1 cells derived
from non-tumorigenic prostate epithelial cell lines. Among the genes analyzed, SNAI2/
Slug was rapidly and strongly upregulated. Changes in expression levels of ZEB1 and
ZEB2 mRNA and miRNA of miR-200 family are observed after extended periods of TGF-ß1
treatment and correlate with E-cadherin expression and progression of EMT but may not
be responsible for rapid upregulation of EMT-driving transcription factors.
Acknowledgements: This work was supported by grants No. 310/07/0961 and 303/09/H048
of the Czech Science Foundation, IGA MZD 9956-4/2008, IGA MZD 9600-4/2008, by the AS
CR, grants no. AV0Z50040507 and AV0Z50040702 and by grant no. CZ.1.07/2.3.00/09.0020.
142 XXII. Biochemistry Congress, Martin