XXII. BIOCHEMICKÝ ZJAZD - Jesseniova lekárska fakulta

Posters
95.
METYLNICOTINAMIDE AND DNA OXIDATION DAMAGE IN raTS WITH
STREPTOZOTOCINE INDUCED DIABETES MELLITUS
Zuzana Országhová 1 , Zuzana Paduchová 1 , Ingrid Žitňanová 1 , Cezary Watala 2 ,
Jana Muchová 1 and Zdeňka Ďuračková 1
1
Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry,
Medical School, Comenius University, Bratislava, Slovakia;
2
Department of Haemostasis and Haemostatic Disorders,
Medical University of Lodz, Poland
Increased oxidative and glycooxidative stresses contribute to the development and progression
of diabetes-associated complications. Under conditions of DM many important
biomolecules including DNA, lipids or proteins are affected. N(1)methylnicotin-amide
chloride (MNA), which is a derivative of nicotinamide, is an intensively studied agent
possessing remarkable anti-inflammatory as well as antidiabetic properties. This effect is
associated with scavenging of reactive forms of oxygen, in particular superoxide radical
anion and hydroxyl radical. In this study we have studied the level of oxidative damage to
DNA in lymphocytes and liver tissue as well as markers of glycation (AGEs) and oxidation
(carbonyls) damage of proteins in streptozotocine induced diabetic rats. Administration
of MNA in dose 200 mg/kg of weight during 7 weeks significantly decreased DNA damage
in lymphocytes and level of protein carbonyls. All doses of MNA decreased DNA damage
in liver tissue. Advanced glycation of proteins were not affected with MNA. Our results
show possible beneficial protective role of MNA against oxidative stress and damage of
important biomolecules under the conditions of DM.
Acknowledgement: This study was partially supported by MVTS and VEGA grants.
XXII. Biochemistry Congress, Martin
219