Scientific Papers Series A. Agronomy

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Halstedii is necessary to identify local populations and monitor their changes over time. MATERIALS AND METHODS To achieve experiments on identifying pathogen races present in sunflower crop in Romania was used following set of differentiators: HAR-304 who is sensitive for all downy mildew races; RHA-265 and RHA- 266 who are resistant for race 100 and sensitive for races 300, 310, 330, 700, 710 and 730 downy mildew races; RHA-274 who is resident for races 100, 300, 330, undefined for 310 race and sensitive for races 700, 710, 730 downy mildew races; PMI 3 who is resistant for races100, 300, 330,700, undefined for 310 races and sensitive for 330, 710 and 730 races; PM 17 who is resistant for races 100, 300, 700, 710, undefined for 310 races and sensitive for 330 and 730 races; 803-1, RHA-340 and HAR- 4 who are resistant for all downy mildew races; HA-335 who is resistant for races 100, 300, 330, 700, 710, 730, undefined for races 310 and sensitive for 304 races; DM-2 who is resistant for races 100, 300, 304, 700 and sensitive for races 330, 710 and 730. To identify the spectrum of races of the pathogen were collected sunflower plants attacked by mildew from following areas cultivated sunflower in the country: Movilita – Ialomita, Tamadau – Calarasi, Peciu Nou – Timis, Farcasele – Olt, Perieti – Ialomita, Mihail Kogalniceanu – Constanta, Ianca – Braila, Dor Marunt – Calarasi. These samples were kept in the freezer and multiplied on the susceptible cultivar “Peredovic” using the radicle inoculation method and then subsequently used to obtain suspensions of zoospores for making artificial infection. Identify races of Plasmopara halstedii study was done in the laboratory, in 2012. RESULTS AND DISCUSSIONS In Romania, the pathogen Plasmopara halstedii evolved in increasing virulence, especially in recent years, during which identified five new races of it. If a very long time, about thirty five years ago, there were only two races of the pathogen in the sunflower crop in Romania, but the pathogen has developed new races. The results obtained (Table 1 and Table 2) show that in Movilita were infected the differentiators HAR-304, RHA-266 and RHA- 266, in Tamadau were infected the differentiators HAR-304, RHA-266, RHA-274, PMI 3, PM 17, DM-2 and DHA-265, in Ianca were infected HAR-304, RHA-266, RHA-274, PMI 3, DM-2 and DHA-265, in Dor Marunt were infected HAR-304, RHA-266, RHA-274, PMI 3, PM 17, DM-2 and DHA-265. Table 1. Results of testing of the sunflower differentials set, for resistance to the pathogen first four location Izolate Differentiators Movilita Tamadau Ianca Dor Marunt Degree of infection (%) HAR-304 78.1 80.4 71.4 79.2 RHA-266 53.5 61.2 55.8 57.1 RHA-274 0.0 52.2 54.5 51.1 PMI 3 0.0 61.5 51.9 64.4 PM 17 0.0 59.3 0.0 52.0 803-1 0.0 0.0 0.0 0.0 RHA-340 0.0 0.0 0.0 0.0 HA-335 0.0 0.0 0.0 0.0 DM-2 4.1 57.4 59.1 62.4 HAR-4 0.0 0.0 0.0 0.0 RHA-265 54.4 56.3 57.2 51.2 Table no. 2 shows that in Peciu Nou were infected differentiators HAR-304, RHA-266, RHA-274, PMI 3, PM 17, DM-2 and DHA- 265, in Farcasele were infected differentiators HAR-304, RHA-266, RHA-274, PMI 3, DM-2 and DHA-265, in Perieti were infected differentiators HAR-304, RHA-266, RHA-274, PMI 3, PM 17, DM-2 and DHA-265, and in Mihail Kogalniceanu were infected differentiators HAR-304, RHA-266, RHA-274, PMI 3, PM 17, DM-2 and DHA-265. The results show that in Romania races 100 and 300 are prezent in all studied locations, race 700 is prezent in Movilita and Tamadau locations, race 730 is prezent in Peciu Nou, Perieti, Mihail Kogalniceanu and Dor Marunt locations, race 310 is prezent in Tamadau, Peciu Nou, Perieti, Ianca and Dor Marunt locations, race 330 is prezent in Tamadau, Perieti and Dor Marunt locations, race 710 is prezent in Tamadau, Peciu Nou, Farcasele and Ianca locations. The sunflower crop in Romania have been identified in recent years several new races of the pathogen Plasmopara halstedii, it developing in a relatively short period about ten 247
years, five new races. New races are present in different areas of southern, western and eastern countries. Table 2. Results of testing of the sunflower differentials set, for resistance to the pathogen last four location Izolate Differentiators Peciu Mihail Farcasele Perieti Nou Kogalniceanu Degree of infection (%) HAR-304 58.2 64.5 52.7 72.2 RHA-266 52.3 58.8 54.9 52.1 RHA-274 56.9 63.1 24.2 54.2 PMI 3 57.1 56.6 67.6 51.8 PM 17 59.8 0.0 55.8 62.0 803-1 0.0 0.0 0.0 0.0 RHA-340 0.0 0.0 0.0 0.0 HA-335 0.0 0.0 0.0 0.0 DM-2 59.1 52.8 54.0 61.2 HAR-4 0.0 0.0 0.0 0.0 RHA-265 55.1 67.2 61.8 56.2 Table 3. The patotypes of the pathogen Plasmopara halstedii, identified in the sunflower crop, in seven locations Location 1 2 3 4 5 6 7 Downy mildew race 100 300 700 730 310 330 710 Movilita x x x Tamadau x x x x x x Peciu Nou x x x x x Farcasele x x x Perieti x x x x x Mihail Kogalniceanu x x x Ianca x x x x Dor Marunt x x x x x CONCLUSIONS The use of hybrids with genetic resistance to new races of Plasmopara halstedii developed in Romania. Using the seed treatment with chemicals (fungicides) that can prevent infection by the fungus Plasmopara halstedii developed. Identifying new sources of resistance and their use in creating new hybrizi resistant to fungus infections. Monitoring the evolution in time and space developed by fungus Plasmopara halstedii races. To achieve the research is essential establish a differentiator assortment created of inbred lines and hybrids with reaction known to the pathogen, with the participation of private companies and research institutes. REFERENCES Albourie J.M., Tourvieille J., Tourvielle de Labrouhe D., 1998. Resistance to metalaxill in isolates of the sunflower pathogen Plasmopara halstedii. European Journal of Plant Pathology, 104, p. 235-242. Dussle C.M., Hahn V., Knapp S.J., Bauer E., 2004. PlArg, from Helianthus argophyllus is unlinked to other known downy mildew resiatance genes in sunflower. Theor. Appl. Genet., 109, p. 1083-1086. Gulya T., Berlin N., Lamey A., 1994. Sunflower disease. In: Sunflower Production Ext. Bulletin. North Dakota. Agric. Experiment Station and North Dakota State Univ., p. 44-62. Gulya T.J., Rashid K.Y., Maširevic S., 1997. Sunflower Diseases, Sunflower Technology and Production. A.A. Schneiter, ed. ASA, CSSA and SSSA, Madison WI, USA, p. 263-379. Gulya T.J., Tourvieille de Labrouhe D., Masirevic S., Penaud A., Rashid K., Virany F., 1998. Proposal for standardized nomenclature and identification of races of Plasmopara halstedii. ISA Symposium II, Sunflower Downy Mildew, Fargo (USA), 13-14 January, p. 130-136. Gulya T.J., 2007. Distribution of Plasmopara halstedii races from sunflower around the world. In: Proc. of 2nd Int. Downy Mildew Symposium, Olomouc, Czech Republic, Palacky University, vol.3, p. 121- 134. Korosi K., Lazar N., Virany F., 2007. Resistance responce to downy mildew (Plasmopara halstedii) in sunflower, activated by chemical inducers. Advances in Downy Mildew Research, vol.3, Palacky University, Czech Republic, p. 237-241. Molinero-Ruiz L., Dominguez J., Melero-Vara J.M., 2000. Evaluatiuon of Spanish isolates of Plasmopara halstedii for tolerance to metalaxill. Helia, 23, p. 33- 38. Pacureanu J.M., Raranciuc S., Stanciu D., 2006. Evolution of the pathogen-host plant relationship, into Plasmopara halstedii (Helianthi)-Helianthus annuus L. system, in Romania. SUNBIO Conference, Gengenbach, Germany, September. Abstracts: 7. Radwan O., Bouzidi M.F., Nicolas P., Mouzeray S., 2004. Development of PCR markers for the Pl5/Pl8 locus for resistance to Plasmopara halstedii in sunflower, from complete CC-NBS-LRR sequences. Theor. Appl. Genet., 109, p. 176-185. Streten T., Bosko D., Jovanka A., Masirevic S., 2006. Transferring of Plasmopara resistance from annual wild into cultivated sunflower. In: Abstracts of 1th Symposium on Sunflower Industrial Uses, Udine, Italy. Vear F., Serre S., Roche S., Walser P., Tourvieille de Labrouhe D., 2006. Recent research on downy mildew resistance useful for breeding industrial use sunflowers. In: 1 st Symposium on Sunflower Industrial Uses, Udine, Italy. 248
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SCIENTIFIC PAPERS SERIES A. AGRONOM
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University of Agronomic Sciences an
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SUMMARY SOIL SCIENCES Variation of
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A survey study on determination of
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Effects of different nitrogen forms
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Determine the effect of some cultiv
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SOIL SCIENCES
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K + /Na + ratio that ultimately pre
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ZINC POLLUTION OF SOILS LOCATED INT
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as the result of the plant protecti
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OBTAINING OF HUMATE-BASED LIQUID FE
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an 11.6% mass loss and a 145.5 J/g
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mijloc important de fertilizare ech
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collected on 13/09/2012 at the dept
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Table 3. The Influence of mineral f
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CONCLUSIONS The Hybrid rapeseedseed
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Scientific Papers. Series A. Agrono
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CONCLUSIONS The results have shown
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organic carbon was determined thoug
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THE INFLUENCE OF SOIL TILLING SYSTE
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emerging plant, we recommend using
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at the cellular level of Ni (Bou et
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concentrations in agricultural soil
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Surfaces at the bottom shows higher
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substantial balance that means maxi
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Table 4. Aggregate composition and
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1. The initial parameters of ordina
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Table 8. Modification of the featur
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Abstract Scientific Papers. Series
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the linear correlation coefficient
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CONCLUSIONS For soil polluted with
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50 and 100 mg/kg and appreciated th
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Figure 4. Copper content in soil Fi
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samples collected in northern of fa
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MATERIALS AND METHODS Study areas T
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influenced by heavy metals contamin
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Pankhurst C.E., 1997. Biodeversity
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horizons, color, texture, structure
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From a chemical reaction these soil
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- Organic matter by Tiurin; - pH (H
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RESULTS AND DISCUSSIONS Invertebrat
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of moisture in arable layers, incre
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experimentation. The increase of mo
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THE TRANSITION PERIOD TO THE MARKET
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Activity data Activity data comes f
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Figure 2. The decrease of cultivate
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of nitrous oxide. In: Revised 1996
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Laboratory results have been interp
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The air-mass interferes with the ov
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Under these conditions, Moldavian r
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decreased to near wilting coefficie
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CONCLUSIONS Severe drought that aff
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(temperature and soil moisture, air
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primary cultures analyzed, hybrids
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weeding crops during the growing se
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improper drainage and irrigation (G
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Figure 4. Scores plot of the soil s
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Table 1. Cluster analysis of the st
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DIFFERENTIATION OF FERTILIZATION RA
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Figure 2. Situation and location of
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Statistical data processing For the
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Phosphorus and potassium fertilizer
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30 m. Regarding the formation and d
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considerably less. These facts are
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Table 2. Hydraulic dimensioning Par
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SPATIAL VARIATION OF PHYSICAL CLAY
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Figure 2. Situation and location of
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esistant to high clay content in th
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CONCLUSIONS Described regularities
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ecome widespread and in order to ea
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Table 3. Results of the analysis of
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COMPARISON OF SYSTEMS FOR TAXONOMY
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RESULTS AND DISCUSSIONS Soil types
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underdeveloped cinnamon forest soil
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Figure 8. Content of skeletal fract
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MODEL FOR INVESTIGATION, AMELIORATI
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RESULTS AND DISCUSSIONS Characteris
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terrain is in the composition of a
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Such characteristics require the ne
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Australia) with high resolution nit
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some important medicinal plants of
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RFV = [120 / NDF] × [88.9 - (0.779
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average at Viani - Brila County). A
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Figure 1. Dry above-ground biomass
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County) and 30.29% (CERA 270 hybrid
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phenological stages were found to h
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STUDIES REGARDING THE INFLUENCE OF
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ainfall were able to have a negativ
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Figure 7. Thermo-hydric stress fact
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In Romania is grown a large assortm
Page 198 and 199: Mainly vitamin C, E and polyphenols
Page 200 and 201: soluble compounds with antioxidant
Page 202 and 203: primarily the water-soluble antioxi
Page 204 and 205: has a split-split-plot design with
Page 206 and 207: Kovács B., Gyori Z., Prokisch J.,
Page 208 and 209: pays off (Mares et al., 2004; Gonz
Page 210 and 211: Table 3. Tested variants V1 Control
Page 212 and 213: about the inner value of flora. The
Page 214 and 215: MATERIALS AND METHODS Biological ma
Page 216 and 217: Figure 2. Correlation between prote
Page 218 and 219: CONCLUSIONS Production and quality
Page 220 and 221: The following presents several tech
Page 222 and 223: 3. Evolution of total weed number i
Page 224 and 225: RESEARCH ON APPLICATION OF NPK FERT
Page 226 and 227: Also increase production significan
Page 228 and 229: In April 2012, the pre-sowing proce
Page 230 and 231: difference with the hoed control sa
Page 232 and 233: RESULTS AND DISCUSSIONS Data about
Page 234 and 235: Table 7. Proved difference between
Page 236 and 237: Scientific Papers. Series A. Agrono
Page 238 and 239: Table 1. Variance analysis experien
Page 240 and 241: Amongst the three precocity groups,
Page 242 and 243: ACKNOWLEDGEMENTS This study was car
Page 244 and 245: to present genetic sources of resis
Page 246 and 247: REFERENCES Antonova T.S., Araslanov
Page 250 and 251: EFFICIENCY OF UTILIZATION OF ASELEC
Page 252 and 253: to separate the effects of genotype
Page 254 and 255: INFLUENCE OF THE CLIMATIC CONDITION
Page 256 and 257: In 2012, at NARDI Fundulea, data fr
Page 258 and 259: parameter from the treated variants
Page 260 and 261: Table 6. The effectiveness of some
Page 264 and 265: Table 1. Probability of the meteoro
Page 266 and 267: At 50% DI in EOF, only a small part
Page 268 and 269: This was probably due to the specif
Page 270 and 271: under deficit irrigation. This prov
Page 272 and 273: RESULTS AND DISCUSSIONS Intervals w
Page 274 and 275: productions from one year to anothe
Page 276 and 277: epresented by soil ecologization an
Page 278 and 279: new created ones as a basic conditi
Page 280 and 281: The highest number of kernels per e
Page 282 and 283: Table 8. Average weight of thousand
Page 284 and 285: CONCLUSIONS The drought from the ye
Page 286 and 287: IWM as appropriate, necessary studi
Page 288 and 289: 100% in the first 6 weeks of growth
Page 292 and 293: locules boll -1 (0.02), seeds locul
Page 294 and 295: Bhutto H., Baloch M.J., Yousaf M.,
Page 296 and 297: Table 4. GCA effects for morpho-yie
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MATERIALS AND METHODS This study wa
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vegetation of each of the swaths, i
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Sudangrass hybrids depending on the
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The parameters of biomass and dry m
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transplanting and ½ applied at fir
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Fertilization Forms Table 2. The me
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growing agricultural farms; evoluti
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In this area, priority must be to p
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soybean seeds are found farms that
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are a better material for haploid i
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Figure 6. Embryo and endosperm mark
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RESEARCHES CONCERNING THE INFLUENCE
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Table 1. The influence of seeding d
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Exagone hybrid. Thus, when sown at
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Table 9. The influence of sowing di
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MATERIALS AND METHODS In order to k
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Table 3. Genotype influence on whea
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Romanian varieties from the experim
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Table 2. Efficacy of some fungicide
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ROMANIAN WHEAT - STRATEGIC PRODUCT
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exceeded 2,000 thou ha, and maximum
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Values over 26% wet gluten were det
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effective and can build a significa
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grassland. This method is commonly
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RESULTS REGARDING DROUGHT RESISTANC
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average for this period is 170.6 mm
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Hybrid FAO grupe Table 2. The main
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length has lowest values at the unt
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CHARACTERISTICS OF SECOND GENERATIO
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developed in Dobrudzha Agricultural
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Table 5. Cytological characteristic
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We can see in the table that all li
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RESULTS AND DISCUSSIONS Mean compar
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Table 4. Statistical interpretation
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COMPARATIVE STUDY OF CONVENTIONAL A
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Improved sunflower hybrids for use
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differences in production to contro
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International Center for Agricultur
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Table 3. Evaluation of Genotypes fo
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Table 1. Characteristics of city sl
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Table 4. Amount of the rainfall dur
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ECOLOGICAL AGRICULTURE
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maize had a density of 5 plants/m 2
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CONCLUSIONS In terms of productivit
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amino acids, such as 4-hydroxyisole
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Table 5. Determining chlorophyll pi
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PROPOSALS ON TECHNOLOGY OF MOVING A
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These are the main technical charac
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FUNGAL BIODIVERSITY AND CLIMATE CHA
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Climate change effects and Dobrogea
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Figure 10. Pure culture of benefici
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MANAGEMENT OF BENEFICIAL MICROORGAN
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microorganisms DSMZ Braunschweig -
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Protocol of research and reporting
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“HERBAL MEDNET” AN INNOVATIVE A
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Figure 2. Value of global herbal ma
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Figure 3. The Allelopathy effect on
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AGRICULTURAL ENGINEERING AND RENEWA
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Research was also aimed at observin
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Leaf production of dry matter per h
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cooking oil and jatropha oil (Vonsh
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machines, many different fat extrac
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Table 4. The nutrient content Conte
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Figure 21. Dunaliella salina sp. ch
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for the decline is the growing numb
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Tractor power seemed the obvious wa
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Figure 4 shows that in 17 post war
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their employability, help raise the
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. Scientific Papers. Series A. Agro
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through the sprinkler irrigation. A
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Figure 5. Overlapping sprinklers wi
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CLIMATE CHANGE AND AGROMETEOROLOGIC
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Bulgaria to the use of agroclimatic
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Figure 9. Duration of the period so
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Figure 18. Evapotranspiration of wi
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RESULTS AND DISCUSSIONS After scari
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The influence of the texture on the
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Figure 6. Area of land planted with
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APPLIED BIOLOGY IN AGRICULTURAL SCI
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MATERIALS AND METHODS Researches we
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RESEARCHES REGARDING EVOLUTION OF S
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gradually reduce towards the end of
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According to that, the aspect of th
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etween classes of variation with ma
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Table 2. Average heights of Lycium
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V 1 V 2 Table 6. Average shoot leng
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Table 13. Average dry substance of
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centrifuge (7000 rev/min. during 20
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7, Streptomyces sp. 9, and Streptom
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laboratory of Biology, USAMV Buchar
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SOME NEW SPECIMENS FOR ZOOLOGICAL T
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New reptiles for our teaching colle
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Figure 1. Scatter plot of odontosty
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Table 4. Measurements of Xiphinema
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Malus pumila and P. avium are new h
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- the mean, minimum and maximum mon
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The monthly mean of the flow highli
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longer in balance with the existing
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ecording the encountered plant spec
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Mediterranean (3), Balkan (1), Cent
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Table 3. Segetal species of the Top
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of sugar/flower and the honey poten
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Territorial concentration of mellif
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CONCLUSIONS In Giurgiu County there
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farm (Awake, 1998). Rahman et al. 2
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Table 3 shows that there was a high
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RESULTS AND DISCUSSIONS x, k, r, R
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Figure 3. Us-Pressure Relations of
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