Scientific Papers Series A. Agronomy

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Figure 2. The presence of bacterial species with degradation potential in experimental variants at different determination moments Frequent bacterial isolated from experimental variants, in most determination stages were: Pseudomonas, Arthrobacter, Mycobacterium, bacterial of the most reputable in terms of species with high capacities in hydrocarbon biodegradation. Isolates of Pseudomonas, Arthrobacter and Mycobacterium led to the establishment of inoculum applied to the soil, and very high frequency showed a better survival rate and their adaptability to environmental conditions. Bacterial isolated as Pseudomonas and Arthrobacter dominated the heterotrophic bacteria population in the majority of experimental variants, occasionally accompanied by species belonging to the genera: Micrococcus, Enterobacter and Flavobacterium. Dynamics of microorganisms inoculated showed a slight adaptability in soil polluted with 5% crude oil, but complete inhibition in the first 30 days of experiment at 10% crude oil. After the acclimatization period by 30 days, the excessive concentration of pollutants has been massive multiplication of bacteria in inoculated variants, especially in the conditioned variant with Ecosol maximum dose. At 45 days, there was a real explosion in variants of bacteria inoculated with THB values double, triple compared with uninoculated variants. At 60 days, bacterial populations have begun to reduce their size, as the value of soil polluted with 10% crude oil and untreated. This demonstrates that the application reduces the time needed to adapt to Ecosol substrate for microorganisms involved in petroleum hydrocarbons biodegradation. The value of the total heterotrophic bacteria (THB) in soil is a response to a large number of factors, including crude oil content, which causes new ecological conditions with a strong impact on microbial evolution. These conditions affect not only the size, but also the diversity of bacterial communities. The soil is mainly colonized by bacterial genera species able to use hydrocarbons from crude oil in its metabolism (Voiculescu et al., 2003). Survival of microorganisms in petroleum hydrocarbons medium in uninoculated variants and after bacterial inoculation in the inoculated variants is a key deciding factor in the rate of biodegradation of hydrocarbons in soils. 65
CONCLUSIONS For soil polluted with 5% crude oil, the bacterial top, including those placed in the soil by inoculation was 30 days after impact, respectively 7 days after inoculum application, while in soil polluted with 10% crude oil, multiplication top of bacteria was observed in the determination made at 45 days after impact and 21 days after inoculum application, showing once again how necessary is for microorganisms habituation and adaptation to environment being a function of pollutant concentration. The microorganisms inoculated showed a slight adaptability in soil polluted with 5% crude oil, but complete inhibition in the first 30 days of experiment at 10% crude oil. After the acclimatization period by 30 days, the excessive concentration of pollutants has been massive multiplication of bacteria in inoculated variants, especially in the conditioned variant with Ecosol maximum dose. The application reduces the time needed to adapt to Ecosol substrate for microorganisms involved in petroleum hydrocarbons biodegradation. REFERENCES Alexander M., 1997. Sequestration and Bioavailability of Organic Compounds in Soil in Linz D.G., and Nakles D.V. (eds.) Environmentally Acceptable Endpoints in Soil, American Academy of Environmental Engineers, USA. Atlas R.M., Bartha R., 1992. Hydrocarbon biodegradation and oil spill, Bioremediation. In: Advances in Microbial Ecology, Marshall K. C (editor), Vol 12. Plenum. New York,.USA, p. 287- 338. Beck A.J., Wilson S.C., Alcock R.E., Jones K.C., 1995. Kinetic constraints on the loss of organic chemicals from contaminated soils: Implications for soil-quality limits, Critical Reviews in Environmental Science and Technology 25, p. 1-43. Burger A.E., 1993. Estimating the mortality of seabirds following oil spills – effects of spill volume. Marine Pollution Bulletin 26, 239–248. Burns K.A., Garrity S.D., Levings S.C., 1993. How many years until mangrove ecosystems recover from catastrophic oil-spills. Marine Pollution Bulletin 26, p. 239–248. Cerniglia C.E., 1992. Biodegradation of polycyclic aromatic hydrocarbons. Biodegradation 3, p. 351-368. Dumitru M, Ru C, Toti M, Gamen E., 1994. Evaluarea gradului de poluare a solului. Msuri de eliminare a efectului poluant, Conferina Naional pentru tiina Solului, Bucureti. Leahy J.G., Colwell R.R., 1990. Microbial degradation of hydrocarbons in the environment. Microbiol. Rev. 54, p. 305–315. Lee K. and Levy E.M., 1989. Bioremediation of petroleum in the marine environment and its enhancement, Aquatic Toxicology Manager, J. A. Nriagu ed., John Wiley and Sons, New York, p. 217-243. Loynachan T.E., Brown K.W., Cooper T.H., Milford M.H., 1999. Sustaining our soils and society, AGI (American Geological Institute) Environmental Awareness, <strong>Series</strong> 2. Nikolopoulou M., Pasadakis N., Kalogerakis N., 2007. Enhanced bioremediation of crude oil utilizing lipophilic fertilizers. ScienceDirect, Desalination 211, p. 286–295. Norris R.D., Matthews J.E., 1994. Handbook of Bioremediation, Lewis Publishers, Boca Raton, Florida. Okoh A.I., Trejo-Hernandez M. R., 2006. Remediation of petroleum hydrocarbon polluted systems: Exploiting the bioremediation strategies. African Journal of Biotechnology Vol. 5 (25), p. 2520-2525. Pepper I.L., Gerba C.P., Brusseau M.L., 1996. Pollution Science. Academic Press, p. 387. Pothuluri J.V., Cerniglia C.E., 1998. Current aspects on polycycyclic aromatic hydrocarbon biodegradation processes. In: Sikdar, S.K., Irvine, R.L. (Eds.), Bioremediation: Principles and Practice, vol. 2 Technomic Publishing Company, Lancaster, p. 461- 520. Rahman K.S.M., Thahira-Rahman J., Kourkoutas Y., Petsas I., Marchant R., Banat I.M., 2003. Enhanced bioremediation of n-alkanes in petroleum sludge using bacterial consortium amended with rhamnolipid and micronutrients. Bioresource Technology 90, p. 159–168. Sadler R., Connel D., 2003. Analitycal Methods for the Determination of Total Petroleum Hydrocarbons in Soil. Proceedings of the Fifth National Workshop on the Assessment of Site Contamination. Snape I., Riddle M.J., Stark J.S., Cole C.M., King C.K., Duquesne S., Gore D.B., 2001. Management and remediation of contaminated sites at Casey Station. Antarctica, Polar record, 37:p. 199-214. Voiculescu A.R., Dumitru M., Toti M., 2003. Decontaminarea Solurilor Poluate cu Compui Organici, Ed. SITECH, Craiova. 66
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SCIENTIFIC PAPERS SERIES A. AGRONOM
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University of Agronomic Sciences an
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SUMMARY SOIL SCIENCES Variation of
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A survey study on determination of
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Effects of different nitrogen forms
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Determine the effect of some cultiv
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SOIL SCIENCES
Page 17 and 18: K + /Na + ratio that ultimately pre
Page 19 and 20: ZINC POLLUTION OF SOILS LOCATED INT
Page 21 and 22: as the result of the plant protecti
Page 23 and 24: OBTAINING OF HUMATE-BASED LIQUID FE
Page 25 and 26: an 11.6% mass loss and a 145.5 J/g
Page 27 and 28: mijloc important de fertilizare ech
Page 29 and 30: collected on 13/09/2012 at the dept
Page 31 and 32: Table 3. The Influence of mineral f
Page 33 and 34: CONCLUSIONS The Hybrid rapeseedseed
Page 35 and 36: Scientific Papers. Series A. Agrono
Page 37 and 38: CONCLUSIONS The results have shown
Page 39 and 40: organic carbon was determined thoug
Page 41 and 42: THE INFLUENCE OF SOIL TILLING SYSTE
Page 43 and 44: emerging plant, we recommend using
Page 45 and 46: at the cellular level of Ni (Bou et
Page 47 and 48: concentrations in agricultural soil
Page 51 and 52: Surfaces at the bottom shows higher
Page 53 and 54: substantial balance that means maxi
Page 55 and 56: Table 4. Aggregate composition and
Page 59 and 60: 1. The initial parameters of ordina
Page 61 and 62: Table 8. Modification of the featur
Page 63 and 64: Abstract Scientific Papers. Series
Page 65: the linear correlation coefficient
Page 69 and 70: 50 and 100 mg/kg and appreciated th
Page 71 and 72: Figure 4. Copper content in soil Fi
Page 73 and 74: samples collected in northern of fa
Page 75 and 76: MATERIALS AND METHODS Study areas T
Page 77 and 78: influenced by heavy metals contamin
Page 79 and 80: Pankhurst C.E., 1997. Biodeversity
Page 81 and 82: horizons, color, texture, structure
Page 83 and 84: From a chemical reaction these soil
Page 85 and 86: - Organic matter by Tiurin; - pH (H
Page 89 and 90: RESULTS AND DISCUSSIONS Invertebrat
Page 91 and 92: of moisture in arable layers, incre
Page 95 and 96: experimentation. The increase of mo
Page 97 and 98: THE TRANSITION PERIOD TO THE MARKET
Page 99 and 100: Activity data Activity data comes f
Page 101 and 102: Figure 2. The decrease of cultivate
Page 103 and 104: of nitrous oxide. In: Revised 1996
Page 105 and 106: Laboratory results have been interp
Page 107 and 108: The air-mass interferes with the ov
Page 109 and 110: Under these conditions, Moldavian r
Page 111 and 112: decreased to near wilting coefficie
Page 113 and 114: CONCLUSIONS Severe drought that aff
Page 115 and 116: (temperature and soil moisture, air
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primary cultures analyzed, hybrids
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weeding crops during the growing se
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improper drainage and irrigation (G
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Figure 4. Scores plot of the soil s
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Table 1. Cluster analysis of the st
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DIFFERENTIATION OF FERTILIZATION RA
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Figure 2. Situation and location of
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Statistical data processing For the
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Phosphorus and potassium fertilizer
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30 m. Regarding the formation and d
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considerably less. These facts are
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Table 2. Hydraulic dimensioning Par
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SPATIAL VARIATION OF PHYSICAL CLAY
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Figure 2. Situation and location of
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esistant to high clay content in th
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CONCLUSIONS Described regularities
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ecome widespread and in order to ea
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Table 3. Results of the analysis of
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COMPARISON OF SYSTEMS FOR TAXONOMY
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RESULTS AND DISCUSSIONS Soil types
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underdeveloped cinnamon forest soil
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Figure 8. Content of skeletal fract
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MODEL FOR INVESTIGATION, AMELIORATI
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RESULTS AND DISCUSSIONS Characteris
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terrain is in the composition of a
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Such characteristics require the ne
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Abstract Scientific Papers. Series
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Australia) with high resolution nit
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some important medicinal plants of
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RFV = [120 / NDF] × [88.9 - (0.779
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Scientific Papers. Series A. Agrono
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average at Viani - Brila County). A
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Figure 1. Dry above-ground biomass
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County) and 30.29% (CERA 270 hybrid
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phenological stages were found to h
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STUDIES REGARDING THE INFLUENCE OF
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ainfall were able to have a negativ
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Figure 7. Thermo-hydric stress fact
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In Romania is grown a large assortm
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Mainly vitamin C, E and polyphenols
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soluble compounds with antioxidant
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primarily the water-soluble antioxi
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has a split-split-plot design with
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Kovács B., Gyori Z., Prokisch J.,
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pays off (Mares et al., 2004; Gonz
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Table 3. Tested variants V1 Control
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about the inner value of flora. The
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MATERIALS AND METHODS Biological ma
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Figure 2. Correlation between prote
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CONCLUSIONS Production and quality
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The following presents several tech
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3. Evolution of total weed number i
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RESEARCH ON APPLICATION OF NPK FERT
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Also increase production significan
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In April 2012, the pre-sowing proce
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difference with the hoed control sa
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RESULTS AND DISCUSSIONS Data about
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Table 7. Proved difference between
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Table 1. Variance analysis experien
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Amongst the three precocity groups,
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ACKNOWLEDGEMENTS This study was car
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to present genetic sources of resis
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REFERENCES Antonova T.S., Araslanov
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Halstedii is necessary to identify
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EFFICIENCY OF UTILIZATION OF ASELEC
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to separate the effects of genotype
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INFLUENCE OF THE CLIMATIC CONDITION
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In 2012, at NARDI Fundulea, data fr
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parameter from the treated variants
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Table 6. The effectiveness of some
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Table 1. Probability of the meteoro
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At 50% DI in EOF, only a small part
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This was probably due to the specif
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under deficit irrigation. This prov
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RESULTS AND DISCUSSIONS Intervals w
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productions from one year to anothe
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epresented by soil ecologization an
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new created ones as a basic conditi
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The highest number of kernels per e
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Table 8. Average weight of thousand
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CONCLUSIONS The drought from the ye
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IWM as appropriate, necessary studi
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100% in the first 6 weeks of growth
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locules boll -1 (0.02), seeds locul
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Bhutto H., Baloch M.J., Yousaf M.,
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Table 4. GCA effects for morpho-yie
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MATERIALS AND METHODS This study wa
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vegetation of each of the swaths, i
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Sudangrass hybrids depending on the
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The parameters of biomass and dry m
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transplanting and ½ applied at fir
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Fertilization Forms Table 2. The me
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growing agricultural farms; evoluti
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In this area, priority must be to p
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soybean seeds are found farms that
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are a better material for haploid i
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Figure 6. Embryo and endosperm mark
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RESEARCHES CONCERNING THE INFLUENCE
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Table 1. The influence of seeding d
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Exagone hybrid. Thus, when sown at
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Table 9. The influence of sowing di
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MATERIALS AND METHODS In order to k
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Table 3. Genotype influence on whea
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Romanian varieties from the experim
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Table 2. Efficacy of some fungicide
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ROMANIAN WHEAT - STRATEGIC PRODUCT
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exceeded 2,000 thou ha, and maximum
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Values over 26% wet gluten were det
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effective and can build a significa
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grassland. This method is commonly
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RESULTS REGARDING DROUGHT RESISTANC
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average for this period is 170.6 mm
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Hybrid FAO grupe Table 2. The main
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length has lowest values at the unt
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CHARACTERISTICS OF SECOND GENERATIO
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developed in Dobrudzha Agricultural
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Table 5. Cytological characteristic
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We can see in the table that all li
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RESULTS AND DISCUSSIONS Mean compar
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Table 4. Statistical interpretation
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COMPARATIVE STUDY OF CONVENTIONAL A
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Improved sunflower hybrids for use
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differences in production to contro
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International Center for Agricultur
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Table 3. Evaluation of Genotypes fo
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Table 1. Characteristics of city sl
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Table 4. Amount of the rainfall dur
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ECOLOGICAL AGRICULTURE
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maize had a density of 5 plants/m 2
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CONCLUSIONS In terms of productivit
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amino acids, such as 4-hydroxyisole
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Table 5. Determining chlorophyll pi
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PROPOSALS ON TECHNOLOGY OF MOVING A
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These are the main technical charac
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FUNGAL BIODIVERSITY AND CLIMATE CHA
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Climate change effects and Dobrogea
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Figure 10. Pure culture of benefici
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MANAGEMENT OF BENEFICIAL MICROORGAN
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microorganisms DSMZ Braunschweig -
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Protocol of research and reporting
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“HERBAL MEDNET” AN INNOVATIVE A
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Figure 2. Value of global herbal ma
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Figure 3. The Allelopathy effect on
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AGRICULTURAL ENGINEERING AND RENEWA
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Research was also aimed at observin
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Leaf production of dry matter per h
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cooking oil and jatropha oil (Vonsh
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machines, many different fat extrac
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Table 4. The nutrient content Conte
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Figure 21. Dunaliella salina sp. ch
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for the decline is the growing numb
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Tractor power seemed the obvious wa
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Figure 4 shows that in 17 post war
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their employability, help raise the
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. Scientific Papers. Series A. Agro
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through the sprinkler irrigation. A
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Figure 5. Overlapping sprinklers wi
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CLIMATE CHANGE AND AGROMETEOROLOGIC
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Bulgaria to the use of agroclimatic
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Figure 9. Duration of the period so
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Figure 18. Evapotranspiration of wi
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RESULTS AND DISCUSSIONS After scari
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The influence of the texture on the
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Figure 6. Area of land planted with
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APPLIED BIOLOGY IN AGRICULTURAL SCI
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MATERIALS AND METHODS Researches we
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RESEARCHES REGARDING EVOLUTION OF S
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gradually reduce towards the end of
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According to that, the aspect of th
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etween classes of variation with ma
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Table 2. Average heights of Lycium
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V 1 V 2 Table 6. Average shoot leng
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Table 13. Average dry substance of
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centrifuge (7000 rev/min. during 20
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7, Streptomyces sp. 9, and Streptom
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laboratory of Biology, USAMV Buchar
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SOME NEW SPECIMENS FOR ZOOLOGICAL T
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New reptiles for our teaching colle
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Figure 1. Scatter plot of odontosty
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Table 4. Measurements of Xiphinema
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Malus pumila and P. avium are new h
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- the mean, minimum and maximum mon
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The monthly mean of the flow highli
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longer in balance with the existing
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ecording the encountered plant spec
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Mediterranean (3), Balkan (1), Cent
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Table 3. Segetal species of the Top
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of sugar/flower and the honey poten
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Territorial concentration of mellif
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CONCLUSIONS In Giurgiu County there
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farm (Awake, 1998). Rahman et al. 2
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Table 3 shows that there was a high
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RESULTS AND DISCUSSIONS x, k, r, R
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Figure 3. Us-Pressure Relations of
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