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122 LEISHMANIA VIRULENCE<br />

the restored gene is physiologically relevant. In<br />

some cases, it may be necessary to ‘reintegrate’<br />

the gene back into the target locus in order<br />

to ensure proper temporal and quantitative<br />

expression.<br />

FURTHER READING<br />

Beverley, S.M. (1991). Annu. Rev. Microbiol. 45,<br />

417–444.<br />

Beverley, S.M. and Turco, S.J. (1998). Trends<br />

Microbiol. 6, 35–40.<br />

Blackwell, J.M. (1996). Parasitology 112, S67–S74.<br />

Borst, P. and Ouellette, M. (1995). Annu. Rev.<br />

Microbiol. 49, 427–460.<br />

Clayton, C.E. (1999). Parasitol. Today 15, 372–378.<br />

Cruz, A.K., Titus, R. and Beverley, S.M. (1993). Proc.<br />

Natl. Acad. Sci. USA 90, 1599–1603.<br />

Cunningham, M.L., Titus, R.G., Turco, S.J. and<br />

Beverley, S.M. (2001). Science 292, 285–287.<br />

Gibson, W. and Stevens, J. (1999). Adv. Parasitol.<br />

43, 1–46.<br />

Gueiros-Filho, F.J. and Beverley, S.M. (1997).<br />

Science 276, 1716–1719.<br />

Myler, P.J. and Stuart, K.D. (2000). Curr. Opin.<br />

Microbiol. 3, 412–416.<br />

Späth, G.F., Epstein, L., Leader, B. et al. (2000). Proc.<br />

Natl. Acad. Sci. USA 97, 9258–9263.<br />

Swindle, J. and Tait, A. (1996). In: Smith, D.F. and<br />

Parsons, M. (eds). Molecular Biology of Parasitic<br />

Protozoa, Oxford: IRL Press, pp. 6–34.<br />

Turco, S.J. and Descoteaux, A. (1992). Ann. Rev.<br />

Microbiol. 46, 65–94.<br />

Turco, S.J., Späth, G.F. and Beverley, S.M. (2001).<br />

Trends Parasitol. 17, 223–226.<br />

MOLECULAR BIOLOGY

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