trans

A. sAP
COOH
[T][T](S/T)(S/T)(S/T)SSEG
Ser/Thr
rich domain
L. mexicana sAP1 gene repeat sequence:
Phosphoglycans
L. donovani sAP-1/sAP-2 gene repeat sequences:
(T/I)T(T/A)SSSEG
[Manα1,2] 0-5 -Manα1-PO 4 -Ser VTTASSSAD
NH 2
(/)Glcβ1,3
[Manα1,2] 0-5 -[Manα1-PO 4 -6Galβ1,4] 1-2 -Manα1-PO 4 -Ser
B. L. major fPPG
Araβ1,2
(/)
Galβ1,3
Gal1,3
(/)
Galβ1,4Manα1-PO 4 6Galβ1,4Manα1-PO 4 [6Galβ1,4Manα1-PO 4 -] n -Galβ1,4Manα1-PO 4 -Ser-
C. L. mexicana aPPG
Manα1-PO 4 -6Galβ1,3
Glcβ1,3
Glcβ1,3
Glcβ1,3
Glcβ1,3Glcβ1,3Galβ1,4Manα1PO 4 -6Galβ1,4Manα1-PO 4 -6Galβ1,4Manα1-PO 4 -6Galβ1,4Manα1-PO 4 -6Galβ1,4
Glcβ1,3
Galβ1,4Manα1-PO 4 -6Galβ1,4Manα1-PO 4 -6Glcβ1,3Glcβ1,3Galβ1,4Manα1PO 4 -Glcβ1,
Glcβ1,3
Glcβ1,3
[Manα1,2] 5 Manα1,PO 4 -6Glc
FIGURE 10.5 Structures of secreted Leishmania glycoconjugates. (A) Acid phosphatase (sAP). The acid phosphatases ar
of Ser/Thr-rich peptides that are heavily glycosylated on serine with phosphoglycan chains, similar to those found on LPG.
sensus sequences of the repeat domains where phosphoglycosylation occurs are shown in the box. (B) L. major
proteophosphoglycan) is a mucin-like molecule with extensive phosphoglycosylation on serine residues. (C)
(amastigote-specific PPG) is distinct from fPPG, and although it shares phosphoglycan structures with LPG and sAP
with unique branched glycans.