Editorial & Advisory Board - Acta Technica Corviniensis

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ACTA TECHNICA CORVINIENSIS – Bulletin of Engineering CONCLUSIONS For an ESP that collects high resistivity dust the presence of back Corona discharges is inevitable problem. The negative resistance area from a current voltage characteristics (if the characteristics depending on minimum voltage) is a result of back Corona discharges. With modern control techniques and with adapted algorithm the back Corona may be detected. The back‐Corona phenomenon is diminish if is burn coal with better characteristics. Another solution to diminish back Corona discharges is to modify the dust resistivity by condition the flue gas (with sulphur and ammonia). Using another control technique, intermittent energisation or pulse energisation will be decreasing the back Corona discharges. REFERENCES [1.] K. Huang – Spark and its Effect on Electrostatic Precipitators, the X th International Conference on Electrostatic Precipitation (ICESP), Cairns, Queensland, Australia, july, 2006, no. 5B1, 12 pp. [2.] W. Wang, R. Li, F. Cao, A. Fang, S. Chen, A. Pei – The Newest Research Results for Collection of High Specific Resistance Dusts with Electrostatics Precipitators, the X th International Conference on Electrostatic Precipitation (ICESP), Cairns, Queensland, Australia, july, 2006, no. 3B2, 11 pp. [3.] G. Bacchiega, I. Gallimberti, V. Arrondel, N. Caraman, M. Hamlil – Back Corona Model for Prediction of ESP Efficiency and Voltage‐Current Characteristics, the X th International Conference on Electrostatic Precipitation (ICESP), Cairns, Queensland, Australia, july, 2006, no. 3B3, 11 pp. [4.] K.R.Parker and other – Applied Electrostatic Precipitation, Chapman and Hall, Londra, U.K., 1997. [5.] J.M.Leach, S.J. Duddy – The Development of an Algorithm for the Dynamic Adjusment of the Pulse Repetition Frequency for Minimising Back Corona in Electrostatic Precipitators, the IX th International Conference on Electrostatic Precipitation (ICESP), Mpumalanga, South Africa, may 17‐21, 2004, 9 pp. [6.] W. Weixue, F. Aimin, C. Feng – The Newest <strong>Technica</strong>l Results Capable of Remarkably Increasing ESP Efficiency, the IX th International Conference on Electrostatic Precipitation (ICESP), Mpumalanga, South Africa, may 17‐21, 2004, 10 pp. [7.] S. Oglesby, G. Nichols – Electrostatic Precipitation, Dekker, New York, U.S.A., 1978. [8.] G. Bacchiega, I. Gallimberti – Static and Dynamic Back‐Corona Characteristics, the IX th International Conference on Electrostatic Precipitation (ICESP), Mpumalanga, South Africa, may 17‐21, 2004, 11 pp. ACKNOWLEDGMENT This paper was supported by the project "Development and support for multidisciplinary postdoctoral programs in primordial technical areas of the national strategy for research ‐ development ‐ innovation" 4D‐POSTDOC, contract no. POSDRU/89/1.5/S/52603, project co‐funded from the European Social Fund through the Sectorial Operational Program Human Resources 2007‐2013. ACTA TECHNICA CORVINIENSIS – BULLETIN of ENGINEERING ISSN: 2067‐3809 [CD‐Rom, online] copyright © UNIVERSITY POLITEHNICA TIMISOARA, FACULTY OF ENGINEERING HUNEDOARA, 5, REVOLUTIEI, 331128, HUNEDOARA, ROMANIA http://acta.fih.upt.ro 96 2012. Fascicule 2 [April–June]
1. K.P. RENUKADEVI, 2. J. ANGAYARKANNI, 3. G. KARUNAKARAN EXTRACTION AND CHARACTERIZATION OF LIPOPOLYSACCHARIDE FROM SERRATIA RUBIDAEA AND ITS CYTOTOXICITY ON LUNG CANCER CELL LINE‐NCI‐H69 1, 3. DEPARTMENT OF BIOTECHNOLOGY, DR.GRD COLLEGE OF SCIENCE, COIMBATORE, TAMILNADU, INDIA 2. SCHOOL OF BIOTECHNOLOGY AND GENETIC ENGINERING, BHARATHIAR UNIVERSITY, COIMBATORE, TAMILNADU, INDIA ABSTRACT: The present study was carried out on Lipopolysaccride from Serratia rubidaea followed by its partial purification, characterization, evaluation of its Cytotoxicity on Lung Cancer Cell Line. LPS was extracted from Serratia rubidaea and detected by the addition of Schiff’s reagent. The various subgroups such as 2‐KDO, uronic acid and 4‐amino arabinose were detected by paper chromatography and the subgroups such as Lipid A and free amino groups were detected by Thin layer chromatography. Partial purification of the extracted LPS was done by SDS‐PAGE in which bands were obtained in ladder like patterns. The presence of fatty acids, esters, phosphates in LPS of S.rubidaea were revealed by GC‐MS analysis. LPS was found to exhibit cytotoxicity on the lung cancer cell line NCI‐H69. KEYWORDS: LPS, Serratia rubidaea, NCI‐H69, Cytotoxicity INTRODUCTION Lipopolysaccharide (LPS) is a major component of the outer membrane contributing greatly to the structural integrity of the bacteria, and protecting the membrane from certain kinds of chemical attack. LPS is an endotoxin, and induces a strong response to animal immune systems. Lipopolysaccharides are of crucial importance to gram negative bacteria, and are therefore candidate targets for new antimicrobial agents. Gram‐negative bacteria produce a variety of polysaccharides that are important in determining plant and animal‐microbe interactions. These polysaccharides include extracellular, capsular and the cell wall lipopolysaccharides (EPSs, CPSs, and LPSs, respectively). Bacterial lipopolysaccharides are commercially important in different industrial area as such as petroleum, textile, pharmaceutical, and fine chemical industry Sanford 1 . Bishop 2 large number of lipopolysaccrides is medically important, since they are immunogenic. LPSs are the major structural component of cells walls of gram‐negative bacteria. They are unique biopolymers whose molecule contains a hydrophobic moiety (lipid A), which consists of fatty acids linked to a phosphorylated disaccharide (comprising two glucosamine residues), and hydrophilic carbohydrate domains. The latter include the core oligosaccharide (the central part of the LPS molecule), consisting of a certain number of monosaccharide residues (approximately10), and O‐ specific polysaccharide chains, consisting of repeated oligosaccharide units, whose structure may significantly vary in different strains of the same species. Serratia a genus of bacteria (tribe Serratieae) gram‐negative, rod which produces a red pigment. Lipopolysaccride exhibit a numerous biological activity as antibiotics, helps in immune response, and anticancer property. Lipopolysaccharide exhibits a cytotoxic action on various types of cells via apoptosis 3‐4. . In the present study LPS were extracted and partial purification were performed from selected species of S. rubidaea. The components present in LPS were analyzed by GC‐MS. The anti cancer property of LPS were determined against lung cancer cell line. MATERIALS AND METHODS GROWTH OF BACTERIA AND EXTRACTION OF LPS The selected Gram‐negative isolate was grown in Brain heart infusion broth Fukushi et al. 5 for 24 hours at 37 o C and the cells were harvested by centrifugation at 5000 rpm for 10 minutes. Extraction of LPS was carried by Westphal and Jann 6 . The bacterial suspension (5 g dry biomass in 100 ml), was mixed with of 90% phenol previously heated at 67 o C, and incubated at 37 o C in an orbital shaker at 160 rpm for 15 minutes. The mixture was then placed on ice to facilitate the separation of phases and centrifuged for 20 minutes at 5000 rpm. The aqueous phase was collected. A second extraction was made on the mixture of phenol and the cellular pellet by addition of distilled water at 67 o C. Both aqueous phases were combined and dialyzed against distilled water until the phenol was completely eliminated. The sample was then clarified by centrifugation at 10,000 rpm for 20 minutes at room temperature to eliminate the insoluble material. LPS was concentrated by alcohol precipitation as follows; Sodium acetate was added at a final concentration of 0.15 M followed by addition of ice cold 96% ethanol drop by drop for a final sample to © copyright FACULTY of ENGINEERING ‐ HUNEDOARA, ROMANIA 97
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ACTA TECHNICA CORVINIENSIS - Bullet
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