Boreskov Institute of Catalysis SB RAS, Novosibirsk, Russia

PP-16INVESTIGATION OF THE KINETICS OF STRAW ENZYMOLYSISEmelyanov V.M., Nurtdinov R.M., Mukhachev S.G., Ablaev A.R.,Valeeva R.T., Gadelshina G.A.Kazan State Technological University, K. Marks Street, 68, Kazan, Russia,phone: +7(843)2314049, fax: +7(843)2314042, e-mail: Emelianov@front.ruOne of the promising renewable resources of plant raw materials are straw andgreen mass of other crops. In Russia, this type of resource is not used efficiently.There are several works in the field of utilization of straw hydrolyzates andenzymolyzates in fodder production and as growth media for the processes ofmicrobiological synthesis. However, enzymatic hydrolysis of cellulose has not yetmastered in industrial biotechnology in Russia.The processes of enzymatic hydrolysis of straw by liquid enzyme preparationsfrom Genencor International PS AO3329-1.OEN GC-440 (with cellulase activity of1470-1800 units/g), PS AO3143-1.1EN Optiflow RC 2.0, PS AO3131-1.OEN GC-220and PS AO3197-1.OEN Acellerase CB100 (with cellulase activity of 6200-7580units/g) are carried out. Model experiments for studying of enzymolysis kinetics arecarried out using paper and cotton as a source of cellulose. Further grinded andsifted through a sieve with 1 mm mesh rye straw was used. Experiments werecarried out at varying liquor ratio and concentrations of enzyme preparations. Strawwas steamed in an autoclave with excess pressure 0,07 - 0,1 MPa for 0,5 - 1 hour.Change in the total content of sugars in hydrolyzate during the process lasting up to8-10 hours is determined. A kinetic model of the process of straw enzymolysis isdeveloped. The values of kinetic constants are determined.105