Boreskov Institute of Catalysis SB RAS, Novosibirsk, Russia

OP-4-5MECHANISM OF ULTRASOUND-ASSISTED ENZYME HYDROLYSISOF CELLULOSEOlga V. Golyazimova, Anatoly A. PolitovInstitute of Solid State Chemistry and Mechanochemistry, Kutateladze, 18,Novosibirsk, 630128, Russia, e-mail: o.golyazimova@gmail.comEnzyme hydrolysis of cellulose is heterogeneous reaction, and to improve thisprocess it is necessary to accelerate mass transfer. To this effect it may be usedultrasound treatment of reagents. As it is known, acceleration of chemical reactionsin ultrasound field may be because of cavitational phenomena. Local growth oftemperature and pressure, appearance of radicals and more rapid mass transfer arein reaction medium because collapses of cavitation bubbles take place. Thisphysicochemical phenomena account for enzyme denaturation and decreasing ofenzyme activity. So it is necessary to study the mechanism of influence of ultrasoundtreatment on enzyme hydrolysis of cellulose.To determine the mechanism of ultrasound-assisted acceleration of enzymehydrolysis, influence of US-treatment on enzyme activity and sorption properties ofcellulose substrate was studied. Also it was found that preliminary ultrasoundtreatment accelerates this enzymatic reaction. It is interesting that ultrasoundtreatment of cellulose in water without enzymes is ineffective, fig. 1 (graph 2). Thepossible mechanism of reaction acceleration by ultrasound is its influence on enzymesorption processes through the acoustocapillary effect.25Conversion, %201510- 1- 2- 3500 1 2 3 4 5 6t, hrsFig. 1. Hydrolysis kinetics of cellulose: 1 – untreated cellulose, 2 – after ultrasoundtreatment without enzymes, 3 – after ultrasound treatment with enzymes.This work was supported by CRDF (RUX0-008-NO-06) and ISTC № 3235 andRussian Academy of Science Presidium Program “Chemical aspects of energy” 19.6.86