Boreskov Institute of Catalysis SB RAS, Novosibirsk, Russia

OP-4-3THE PRACTICAL APPLICATION OF MECHANICALLY ACTIVATEDENZYMATIC HYDROLYSISBychkov A.L. 1,2 , Lomovsky O.I. 11 Institute of Solid State Chemistry and Mechanochemistry, Kutateladze, 18,Novosibirsk, 630128, Russia, e-mail: bychkov.a.l@gmail.com2 Research and Education Centre «Molecular Design and Ecologically SafeTechnologies», Novosibirsk State University, Pirogova, 2, Novosibirsk, RussiaIt’s known that mannanooligosaccharides (MOS) are a perspective replacementfor antibiotics in animal husbandry [1, 2]. Modern technologies of MOS-containingpreparations production are very complicated and require big inputs. In this work theuse of mechanical activated enzymatic hydrolysis to get the antibacterial preparationcontaining mannanoproteins and mannanooligosaccharides is examined. Theprocesses effectiveness from the point of view of bioavailability ofmannanoligosaccharides for chemical and biochemical processes was estimated.The morphological changes of cell wall which take place during mechanical treatmentand enzymatic hydrolysis were studied.As a result of mechanical activation of yeast biomass the reactivity of cell wallpolysaccharides increases in relation to enzymatic hydrolysis. That effect is based oncell wall supramolecular structure disordering. Due to this the diffusion limitations arecancelled and the effectiveness of enzymatic hydrolysis of structural formingcomponent (β-glucan) increases considerably. The combination of mechanicaltreatment and further enzymatic hydrolysis allows to significantly increase (2,9 times)the bioavailability of yeast mannanoligosaccharides.The introduced approach seems perspective in the area of prophylacticantibacterial preparations production for poultry keeping. The preliminary resultswhich were achieved are the evidence of successful use of the product.References:[1]. A.E. Wold, J. Mestecky, M. Tomana, et al. // Infection and Immunity, 58:9 (1990) 3073-3077.[2]. Future of growth-promoting factors. European Lecture Tour, February 13 – March 5, 2006Available from: www.alltech com. Accessed January 15, 2010The research was done with the support of CRDF grant № RUX0-008-NO-06/BP4M08.83