12.07.2015 Views

BP Singh

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170 Shamsher <strong>Singh</strong> and R.K. KhetarpalDouble stranded RNA (ds RNA) analysis is a rapid tool thatcan supplement the information obtained from. bio-assays. Analysisof dsRNA is based on the isolation of disease specific dsRNAs fromvirus infected tissues and their electrophoretic separation on a gel,which is then stained and viewed. However, the presence of nonviraldsRNAs in healthy plants and the apparent absence of dsRNA profile in some viruses, may result in false negatives or falsepositives. Negative dsRNA tests should be confirmed by othermethods before plant material is indexed as virus free.

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