BP Singh

190 B.R. VermaThe technique such as X-ray micrography or projection microradiographyneed to be developed so that radiograph of smallseeds could be enlarged and accurately analysed.6. Simple flotation methodIt has been reported by Kurdjumove (1913) that dry seeds offorest trees infested with phytophagous chalcidoids can beseparated from uninfested dry seeds by simple flotation method.This method was standardised (Verma, 1986) and modified forSesbania green seeds infested with immature stages of seed chalcid,Bruchophagus mellipes Gahan. Infestation can be detected bysplitting open the seed, removing the submerged ~eeds from waterand dissecting them carefully under binocular. The seeds removedfrom the bottom of container contain mostly eggs and insubsefluent removing, seeds may contain larvae, pupae and deadadults. (Verma, 1986).7. Post entry quarantine detection methodsInsects like-capsids (mirids), psyllids, cicadellids, cecidol1)yids,eurytomids, eriophyoids, borers etc. may not be detected at thetime of laboratory examination because (i) eggs/larvae may befeeding deep inside the plant tissue, (ii) perishable nature ofpropagating material makes it difficult for a detailed specializedexamination in a short time and (iii) this type of material representsmost serious quarantine risk Therefore, such type of material maybe detained to allow the eggs to hatch. Suspected plant materialis grown isolated in glass houses/net houses/screen houses andinspected regularly. Plant material may also be grown in thirdcountry (Intermediate Quarantine) where particular host is notavailable for cultivation. Regular survey in the post-entry quarantine(PEQ) nursery is required. As and when the symptoms developand identification of the emerged adult stages is completed, theplanting material may suitably be treated, destroyed or releasedto the indentor. In some cases material is grown for one seasonand healthy produce of the crop is released to the indento~.8. Processing of interceptionsFor adequate detention, control and regular work rapid identificationcan not be relegated to be handled as some time later. Once theinfestation is detected, the identity of the pest needs to beestablished speedily to determine the quarantine risk and the