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INFLUENCE OF N-6-BENZYLADENINE ON THEMICROPROPAGATION OF LAMIUM ALBUM L.M. Dimitrova, Zh. Yordanova, V. Kapchina-TotevaDepartment of Plant Physiology, Faculty of Biology, University of Sofia, 8 DraganTzankov Blvd., 1164 Sofia, Bulgaria; E-mail: veneta@biofac.uni-sofia.bgLamium album L. is a species belonging to the family Lamiaceae that comprisesabout 3500 species of annuals and perennials distributed in Europe, Asia andAfrica. Lamium album L. is herbaceous perennial plant growing up to 50-100 cmand having a green, four-angled stem. Lamium album L. possesses astringent, antiinflammatory,antispasmodic, antibiotic and bacteriostatic properties. Differentconcentration of BA (0,1-1,0 mg.l -1 ) affected micropropagation and callusogenesisand some physiological features such as number and length of shoots, dry and freshweight. The aim of the present study was to investigate the influence of differentconcentration of cytokinin N 6 -benzyladenine (BA) on in vitro propagation ofLamium album L. Explants were inoculated on basal MS media containing 3 %(w/v) sucrose, supplemented with different concentration of N 6 -benzyladenine(BA) in a range of 0,1-1,0 mg.l -1 . The plant material was obtained after four weeksof cultivation. The amount of total phenol was determined by the method of Sweinand Hills. The application of higher concentration of BA (0,6-1,0 mg.l -1 ) decreasedthe length of shoots, number of leaves and dry weight and often leads tovitrification. Callusogenesis, on the other hand, was stimulated in all of theobserved concentrations of BA. The maximum induction of length of shoots,number of leaves and dry weight were achieved from medium supplemented with0,2 mg.l -1 BA with an average shoot length of 4.59 ±1,26 cm and the highest leavesnumber (12,21±1,75). In our model system the application of BA increased theamount of total phenols. In conclusion we assume that the concentration of BA(0,1-0,5 mg.l -1 ) increased the number of leaves, length of shoots and dry weight.BA influenced total phenols of micropropagated Lamium album L.Acknowledgement: This work was financially supported by grant № DTK-02-29/2009 of Ministry of Education, Bulgaria.18ЖИЗНЕНОСТ НА ПОЛЕНА И СЕМЕНАТА НА ARNICA MONTANA(ASTERACEAE) ВЪВ ВРЪЗКА С РЕАЛИЗАЦИЯТА НАРЕПРОДУКТИВНИЯ Й ПОТЕНЦИАЛE. Янкова-Цветкова, Г. Балджиев, П. Юрукова-Грънчарова, А. ВитковаИнститут по ботаника, БАН, ул. „Акад. Г. Бончев”, Бл. 23, 1113 София;E-mail: georgibaldjiev@gmail.comArnica montana е многогодишно растение с розетка, защитено в Европа. То есъс статус на рядък и защитен вид поради интензивното му събиране вприродата. Видът се събира за медицински нужди, тъй като активните мусъставки имат подчертано антибактериално и противовъзпалително действие.108