Програма на научната конференция - Министерство на ...

EVALUATION OF OROBANCHE SECT. OROBANCHE SUBSECT.MINORES IN BULGARIA USING ISSR MARKERSK.H.Stoyanov 1 , E.L. Hristova 2 , I.D.Denev 21 Department Botany, Agricultural University - Plovdiv, 12 Mendeleev Str.,4000 Plovdiv, orobanche@abv.bg; 2 Plant Physiology and Molecular BiologyDepartment, University of Plovdiv, 24 Tsar Assent St., 4000 Plovdiv,iliden@uni-plovdiv.bgThe family Orobanchaceae consists of parasitic species. One groups oftaxonomically problematic species is sect. Orobanche subsect. Minores (Beck)Teryokhin. The species in this subsection (except O. crenata) have been oftenassigned to agg. O. minor because of the great morphological similarity. The aimof this was to find useful molecular makers for characterization of the biodiversityof Orobanche subsect. Minores in Bulgaria and their phylogenetic relationships.Five species of subsect. Minores were examined: O. amethystea Thuill.; O. esulaePančić var. bulgarica T.Georgiev, O. pubescens d’Urv. O. loricata Rchb. и O.crenata Forssk. The DNA was isolated by flowers and amplified using PCR with11 ISSR primers. The ISSR products were separated by gel electrophoresis, themolecular sizes of the bands were calculated, then rearranged in Boolean matricesand recalculated using cluster analysys. The consequent cladogram, based on theaverage Euclidean distances, displayed grouping by species and populations,independently of the host species. The studied samples showed high level ofdispersion and could be separated to genotype groups. The lack of grouping by thehosts demonstrates the independence of the method to the environmentalconditions. This study confirms that the assignment of the small-flowered speciesin agg. O. minor have only practical value and its not genetically justified. In spiteof the big morphological similarity, the representatives of agg. O. minor areindependent species, and not phylogenetically outlying from O. crenata. The studyconfirms the independence of the Balkan endemic species O. esulae. The studyillustrates the high accuracy of the method and its sensivity to differenttaxonomical levels.93