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DIVERSITY OF PHYTOPATHOPGENIC BACTERIA OF THE GENUSBURKHOLDERIA IN BULGARIAY. Kizheva 1 , P. Hristova 1 , N. Bogatzevska 2 , S. Tishkov 1 , V. Chipeva 1 ,P. Moncheva 11 Biological Faculty, Sofia University “St. Kliment Ohridski”, 1164 Sofia,Bulgaria; 2 Plant Protection Institute, Kostinbrod, BulgariaThe aim was to study species and intraspecies diversity of bacteria of the genusBurkholderia isolated from onion bulbs in Bulgaria. Microbiological and molecularmethods were used for the aim realization. The strains were isolated from infectedplant material. The type cultures of Burkholderia gladioli pv gladioli, Burkholderiagladioli pv alliicola, Burkholderia cepacia as well as clinic isolates of B. cepaciawere used as controls. The identification of the pathogenic strains was performedby BIOLOG system and three species were recorded: B. cepacia, B. gladioli and B.pyrrocinia. The intraspecies diversity was studied on the basis of the metaboliteprofile of the strains, obtained by BIOLOG using cluster analysis. The strains wereseparated into seven clusters, which showed the species and intraspecies diversity,which was greater among the strains of B. gladioli. The clinic isolates of B.cepacia distinguished from the phytopathogenic ones. The RFLP-analysis wascarried out with 16S rDNA amplified fragment by the pair of universal primers (9fand 1542r) and endonucleases AluI, TasI and TaqI. The selected enzymesdiscriminated B. cepacia from B. gladioli as well as the two pathovars of B.gladioli. The enzyme AluI revealed polymorphism among the B. gladioli pvgladioli strains. This analysis did not confirmed species belonging to the strainsidentified as B. pyrrocinia by BIOLOG. This study is the first phenotypic andgenotypic characterisation of B. cepacia and B. gladioli in Bulgaria and suggests apolyphasic approach to differentiation of the two pathovars of B. gladioli andintrasepcies diversity. This study was supported by the National ScientificFoundation Project ВУ-Б-206/06.10РАЗНООБРАЗИЕ НА РАСТИТЕЛНО ПАРАЗИТНИ НЕМАТОДИ ОТРОД XIPHINEMA В БЪЛГАРИЯВ. Пенева, С. Лазарова, М. ЕлшишкаЦентрална лаборатория по обща екология – БАН, ул. „Ю. Гагарин” 2,1113 София, България; E-mail: vpeneva@ecolab.bas.bgПредставени са данни за разнообразието на ектопаразитните растителнинематоди от род Xiphinema в България. Те са свързани с широк спектър отрастителни видове. Българската фауна е представена от 13 вида. Сред тях петвида са членове на групата Xiphinema americanum, които са морфологично игенетично различни от останалите видове в рода, разглеждани катопредставители на групата Xiphinema non-americanum; в България сеустановени осем вида от последната група:97