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DOES THE EFFECT OF LILIUM CANDIDUM L. EXTRACT DEPEND ONTHE EXPERIMENTAL DESIGN?L. Hadjo 1 , B. Yankulova 1 , M. Kopásková 2 , P. Mučaji 3 , E. Miadoková 2 ,S.G. Chankova 11 Central Laboratory of General Ecology, Bulgarian Academy of Sciences,2 Gagarin Street, 1113 Sofia, Bulgaria;2 Department of Genetics, Comenius University, 84215 Bratislava,Slovak Republic;3 Department of Pharmacology and Botany, Comenius University, 83232Bratislava, Slovak Republic; E-mail: Stephanie.chankova@yahoo.com;linahadjo@yahoo.com; b_yankulova@abv.bgNowadays a lot of hazardous substances are present in the environment. One verypromising strategy to overcome their harmful influence is antimutagenesis. Theaim of this study is to clarify whether Lilium candidum L. extract (LE) affectsDNA-damaging action of zeocin. We used the CW15 (+) strain of Chlamydomonasreinhardtii L. (lacking cell wall) due to its typical plant cell structure and haploidgenome. Both induced double strand breaks (DSB DNA) and rejoining capacitywere measured according to Chankova and Bryant (2002) and Chankova et al.(2005). Four experimental schemes were designed. No DNA-damaging action wasshown after increasing concentration treatment with LE. Additive effect wasobtained after combined treatment with LE and zeocin. Pretreatment with LE didnot increase the level of DSB DNA induced by zeocin. Interestingly, the protectivepotential of LE was revealed when LE treatment was given 4 hours before zeocintreatment. Acceleration of DSB DNA rejoining was found. This indicated that splittreatment with LE and zeocin could induce adaptive response. It could beconcluded that the effect of the natural product Lilium candidum L. extract doesdepend on the experimental design. Its action varies from no significant effect toadditive or protective effect. Acknowledgement: This study was funded under theprojects BG-SK/206/2009 and SK-BG-0017-08.117