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MENDELNET 2013INTRODUCTIONThe content of titan is low in the majority of plants and usually varies between0.1 – 10.0 mg.kg -1 of the dry matter (Tlustoš P. et al., 2005). In the 20 th century it was discoveredthat its usage may increase the crops. Simultaneously it was discovered that its application caused ahigher activity of certain enzymes (peroxidases, catalases and nitrate reductase) and a highercontent of lypoxigenase and phosphofructokinases (Pais I. 1983; Simon L. et al., 1988; Balík J. etal. 1989). As a result of these findings, a more detailed research of the effect of titan on plants waslaunched (Kužel S. et al., 2003; Alcaraz-Lopez C. et al., 2003; Tlustoš P. 2005). In spite of multiplepositive findings concerning the effects of titan on the phytomass of grown plants, negative findinghave been recorded as well (Hara T., et al., 1976; Walace A., et al., 1977). The aim of this paper isto assess the influence of four doses of fertilizer containing titan applied in two variousconcentrations on the production of aboveground as well as underground phytomass, changes inchlorophyll dynamics and the production of winter wheat crops.MATERIAL AND METHODSThe effect of the Mg-Titanit fertilizer (MGT) was examined by means of a small-area fieldexperiment performed in 2012 in cultivated chernozem (48° 42' N, 17° 70' E). The winter wheat ofthe Šarlota sort was constituted as the model crops. The area of one parcel was 20 m 2 . The Mg-Titanit fertilizer contained 8.5 g titan within 1 litre of fertilizer, 3% magnesium, 4% sulphur,whereas the titan was present in form of titanium ascorbate and the sulphur and magnesium in formof magnesium sulphate (MgSO 4 ). The agrochemical soil parameters are shown in Table 1 whichalso describes the methods of their determination.Table 1. The agrochemical soil parameters before the commencement of the experimentsDepth+N–NH 4-N–NO 3 N an P K Ca Mg S N t C ox(m)mg.kg -1 %pH KCl0.0 – 0.3 10.00 15.20 25.2 73.8 265 6 550 335 7.6 1 617 1.39 7.080.3 – 0.6 9.60 9.70 19.3 51.3 235 7 300 373 9.4 1 533 1.48 7.150.0 – 0.6 9.80 12.45 22.25 62.55 250 6 925 354 8.5 1 575 1.44 7.12Nan – inorganic nitrogen; Nan = N – NH + 4 + N – NO - 3 ; N – NH + 4 - colorimetrically using theNessler agent; N – NO3- - colorimetrically using phenol 2,4-disulfonate acid; P - colorimetrically(Mehlich III - Mehlich, 1984); S - soluble sulfur, spetrometrically ICP after extraction with waterin the ratio 1:5; Nt – via distillation (Kjeldahl - Bremner, 1960); pHKCL – potentiometrically inthe extract of 1.0 M KCL, (Fiala et al., 1999); Cox – oxidometrically (Ťjurin, 1966)The experiment consisted of five variants. 0 – the test variant without application of MGT fertilizer;2xTi 0.2 – double MGT spraying amounting to 0.2 l.ha -1 ; 3xTi 0.2 – triple MGT spraying amounting to0.2 l.ha -1 ; 2xTi 0.4 – double MGT spraying amounting to 0.4 l.ha -1 ; 3 x Ti 0.4 – triple MGT sprayingamounting to 0.4 l.ha -1 . The fertilizer was applied in spring in two, more precisely in three growthstages (BBCH 29, BBCH 32, BBCH 55). The dates of sampling for the aims of analysis andevaluation of aboveground and underground plant phytomass dynamics as well as dynamics ofchanges in the total content of chlorophyll are shown in Table 2.Table 2. The dates and growth stages of crop samples and MGT fertilizer sprayingType of actionsampleNo. 1Ti-sprayingNo. 1sampleNo. 2Ti-sprayingNo. 1sampleNo. 3Ti-sprayingNo. 1sampleNo. 4Date27. III. 12. IV. 3. V. 3. V. 21. V. 21. V. 13. VI.Growth stageend of tilleringBBCH 29end of stem elongationBBCH 32 – 37ear emergenceBBCH 55end of floweringBBCH 67 – 69163 | P age