Untitled - MendelNet 2013 - Mendelova zemědělská a lesnická ...

MENDELNET 2013Fig. 1 MALDI/TOF mass spectra protein fingerprints for the identification of non-resistant S.aureus and resistant strains of S. aureus. Data were collected in the m/z 2000-20000 range afterprocessing 1 ml of S. aureus and the results were compared with a library of software MALDI-TOF. (A) Control strain S.a. identified. (B) S. aureus with addition of 950 µM of Cu 2+ . (C) S.aureus with addition of 950 µM of Zn 2+ . (D) S. aureus with addition of 950 µM of Pb 2+ . (E) S.aureus with addition of 950 µM of Cd 2+ . (F) S. aureus with addition of 350 µM of Ag + . The resultswere compared with a library of software MALDI BioTyper TM 3.1 Version, were completed withphotos of crystals.CONCLUSIONSIn this experiment we have compared the resistant strains of S. aureus formed by the action ofheavy metal ions (Ag, Cu, Cd, Pb and Zn) with non-resistant culture. Results pointed at thesignificant changes in biochemical properties between resistant and non-resistant strains of S.aureus. The obtained results can be used for understanding the changes closely associated with theformation of resistance under the influence of heavy metal ions. These results may serve as a basefor further molecular biologic analyses following up a development of resistance in differentbacterial cultures.REFERENCESAhsan, N., J. Renaut, et al. (2009). "Recent developments in the application of proteomics to theanalysis of plant responses to heavy metals." Proteomics 9(10): 2602-2621.Baker, J., S. Sitthisak, et al. (2010). "Copper Stress Induces a Global Stress Response inStaphylococcus aureus and Represses sae and agr Expression and Biofilm Formation." Applied andEnvironmental Microbiology 76(1): 150-160.Duffy, J., G. Dumyati, et al. (2013). "Community-onset invasive methicillin-resistantStaphylococcus aureus infections following hospital discharge." American journal of infectioncontrol 41(9): 782-786.Elased, K. M., D. R. Cool, et al. (2005). "Novel mass spectrometric methods for evaluation ofplasma angiotensin converting enzyme 1 and renin activity." Hypertension 46(4): 953-959.746 | P age