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Untitled - MendelNet 2013 - Mendelova zemědělská a lesnická ...

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MENDELNET <strong>2013</strong>THE EFFECT OF HEAVY METAL IONS ON STAPHYLOCOCCUSAUREUSChudobova D. 1 , Dostalova S. 1 , Ruttkay-Nedecky, B. 1, 2 , Merlos Rodrigo M.A. 1 ,Adam V. 1, 2 , Kizek R. 1, 21 Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel Universityin Brno, Zemedelska 1, 613 00 Brno, Czech Republic2 Central European Institute of Technology, Brno University of Technology, Technicka3058/10, 616 00 Brno, Czech RepublicE-mail: dagmar.chudobova@centrum.czABSTRACTOur objective was to determine the effect of heavy metal ions on resistant strains of Gram-positivebacterial strain of Staphylococcus aureus using mass spectrometry. The resistant strains of S.aureus were prepared using the nitrate solutions of metals (Ag, Cu, Cd, Zn and Pb). MALDI-TOFmass spectrometry was used for observation the changes in the protein composition in the cell walland also for the determination and identification of the strains using the database MALDI Biotyper.Results obtained from analysis with resistant strains were compared with sensitive control strain ofS. aureus. We observed alterations in S. aureus protein composition pointing at resistancedevelopment under influence of heavy metals ions. Our results develop the possible option ofanalysis of resistant strains and may serve as a support for the monitoring of changes in geneticinformation in resistant strains.Key words: S. aureus, resistance, heavy metals, mass spectrometryAcknowledgments: Financial support by NanoBioMetalNet CZ.1.07/2.4.00/31.0023 andPGS11_2012 is highly acknowledged.743 | P age

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