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Untitled - MendelNet 2013 - Mendelova zemědělská a lesnická ...

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MENDELNET <strong>2013</strong>DETERMINATION OF OIL CONTENT IN WHOLE MILK THISTLESEEDS USING FOURIER TRANSFORM NEAR – INFRARED (FT –NIR) SPECTROSCOPYKoláčková P., Šišperová E.Department of Crop Science, Breeding and Plant Medicine, Faculty of Agronomy,Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech RepublicE-mail: pavla.kolackova@mendelu.czABSTRACTOur objective was to create a calibration model for rapid determination of the oil in whole MilkThistle seeds using the method Fourier Transform Near - Infrared (FT-NIR) Spectroscopy. Theseeds from different distributors and dealers from several European countries and from the harvests2010, 2011 and <strong>2013</strong> were processed by Soxhlet extraction in Det-Gras N instrument and its FT-NIR spectra were collected. The calibration model was created using Partial Least Square (PLS)chemometric analysis – and was successfully tested with the validation samples (spectra notincluded in the calibration model).Key words: milk thistle, rapid determination, seed oil, Fourier Transform Near - Infrared (FT-NIR)Spectroscopy, soxhlet extractionAcknowledgments: This work was supported by Internal Grant Agency of Mendel University inBrno, Czech Republic, No. TP 4/<strong>2013</strong>.649 | P age

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