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Untitled - MendelNet 2013 - Mendelova zemědělská a lesnická ...

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MENDELNET <strong>2013</strong>POLYMORPHISM IN ASIP, MC1R AND MATP GENES IN RELATIONTO COLOR IN HORSESHorecká E., Knoll A.Department of Animal Morphology, Physiology and Genetics, Faculty of Agronomy,Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech RepublicE-mail: eliska.horecka@mendelu.czABSTRACTThe basic color in horses, such as black, brown and chestnut is affected by only two genes MC1Rand ASIP. Other colors in horses are affected by modifying genes, such as dilution gene MATP.In this thesis genotypes of 130 horses were determined using PCR-RFLP for MATP (membranetransport protein association) gene and multiplex PCR-RFLP for MC1R (melanocortin 1 receptor)and ASIP (agouti signaling protein) genes. Subsequently, the allele and genotype frequencies weredetected in a group of horses of different breeds using SAS programe to assess the associationbetween polymorphisms in the MATP gene and phenotype. Statistically, it was evaluated thatthe genotype was highly significantly associated with phenotype.Key words: horse, coloring, MATP, MC1R, ASIPAcknowledgments: This project was supported by CEITEC CZ.1.05/1.1.00/02.0068726 | P age

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