Untitled - MendelNet 2013 - Mendelova zemědělská a lesnická ...

MENDELNET 2013GENOMIC ANALYSES OF DIHYDROFLAVONOL REDUCTASEGENE IN GENOTYPES OF COMMON WHEAT (TRITICUMAESTIVUM L.) WITH NONSTANDARD COLOURED CARYOPSESŠtiasna K. 1 , Sekanina P. 1 , Vyhnánek T. 1 , Trojan V. 1 , Bartoš J. 2 , Havel L. 11 Department of Plant Biology, Faculty of Agronomy, Mendel University in Brno,Zemedelska 1, 613 00 Brno, Czech Republic2 Centre of Plant Structural and Functional Genomics, Institute of Experimental BotanyAS CR, Šlechtitelů 31, 783 71 Olomouc-Holice, Czech RepublicE-mail: xstiasna@node.mendelu.czABSTRACTAnthocyanins are responsible for the coloration in shades of blue, purple and red of various bodyparts of plants. Wheat caryopses with nonstandard coloration, specifically genotypes UC66049 andTschermaks Blaukörniger Sommerweizen with blue aleurone, ANK28B and Abyssinskaya arraseitawith purple pericarp were used in the experiment. Genotype Novosibirskaya 67 was used as astandard because it does not synthesize any pigments. Total RNA was isolated from caryopses bythe phenol-chloroform method and transcribed into cDNA. In dihydroflavonol reductase (DFR)gene sequences obtained from direct sequencing of PCR product were detected several indels andsingle nucleotide polymorphisms. The similarity among all sequences of analyzed genotypes andsequence obtained from National Center for Biotechnology Information (NCBI) ranged between94.59 to 100 %. The gene expression of DFR in samples varied during maturation. For the controlgenotype Novosibirskaya 67 there was highest DFR gene expression 25 days post anthesis (dpa)and the lowest 15 and 35 dpa. In Tschermaks Blaukörniger Sommerweizen was observed thelowest gene expression 40 dpa and the highest 10 dpa, while in genotype UC66049 was DFR geneexpression almost the lowest 10 dpa and 40 dpa reached the lowest values. The highest DFR geneexpression in Abyssinskaya arraseita was observed between 15 and 20 dpa and then rapidlydecreases to its lowest level 25 dpa. Genotype ANK28B shows decreasing gene expression duringmaturation, the highest value was observed 10 dpa and the lowest 35 dpa, 40 dpa gene expressionslightly increased again.Key words: wheat, Triticum aestivum L., anthocyanins, dihydroflavonol reductaseAcknowledgments: This work was supported by IGA FA MENDELU IP No. 12/2013.678 | P age