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MENDELNET <strong>2013</strong>THE EFFECT OF STAPHYLOCOCCUS AUREUS BACTERIATO PROPORTION OF GAMMA DELTA T-LYMPHOCYTESFROM BOVINE MAMMARY GLANDŠustrová T., Sláma P.Department of Morphology, Physiology and Animal Genetics, Faculty of Agronomy,Mendel University in Brno, Zemedelska 1, 613 00 Brno, Czech RepublicE-mail: terez.sustrova@seznam.czABSTRACTThe aim of this thesis was study of lymphocytes, especially subpopulationγδ T-lymphocytes. The experiment was conducted on five clinically healthy heifers. Heifers werecrossbred Holstein and Czech Pied. Their mammary glands were experimentally infected bybacteria Staphylococcus aureus. Bacterial suspension was applied in amout of 5 ml andthe concentration was 800 CFU/ml. Application of inoculum was designed after control lavage withphosphate buffered saline (PBS). Samples of cell populations were obtain by lavage of themammary gland in 4 intervals (24, 48, 72 and 168 hrs) after initiation of experirimental infection.Percentage of lymphocytes and subpopulation γδ T-lymphocytes were detected by flow cytometry.Stimulation of mammary gland by S. aureus led to percetnage increase of lymhocytes from 9.62%detected in 24 hrs to 27.36% in 168 hrs after aplication of inoculum. Percentage increaseof γδ T-lymphocytes was detected too. 24 hrs after stimulation by S. aureus there was 10.5% γδ T-lymphocytes detected out of the total number of lymphocytes. Proportion of γδ T-lymphocytescontinually increased up to 16.46% in 168 hrs. The results show that γδ T-lymphocytes play animportant part in inflammatory response to Gram positive bacteria in heifers´s mammary gland.Key words: γδ T-lymphocytes, Staphylococcus aureus, mammary gland, mastitis, heiferAcknowledgments: I would like to thank Ing. P. Sláma, Ph.D. for leading the diploma thesis,giving valuable advice and sharing his experiences.788 | P age
MENDELNET <strong>2013</strong>INTRODUCTIONMammary gland is protected by many nonspecific and specific defensive substances andmechanisms and by its anatomical structure. Lymphocytes and macrophages are cells which protectmammary gland against pathogens. They are prevalent population of resident cells. During aninfection, recruitment of leukocytes is induced, especially neutrophils from blood. (Miller et al.,1991).γδ T-lymphocytes in adult cows represent 10 – 25 % of circulating T-lymphocytes . (Toman et al.,2009). Wilson et al. (1996) report that this number can be up to 40 % at calves. Davis et al. (1996)report that this number can increase up to 60 % at calves. T-cells expressing γδ TCR are calledunconventional, innate or transient T- cells. That is because these cells have both features – innateas well as specific immune response (Guznam et al., 2012). γδ T-lymphocytes have been shown torecognize non-peptide antigens, pathogen-associated molecular patterns (PAMPs) or dangerassociatedmolecular patterns (DAMPs). These cells appear to be „pre-activated“ as shown by theexpression of memory and activation markers and this may enable rapid induction of effectorfunctions (Boneville et al., 2010). It has been recently shown that the interaction of MIC (proteinswhich are expressed in response to cellular stress) and NKG2D immune receptor (Natural Killergroup 2D) expressed in bovine W1 + γδ T-cells induces their activation in the absence of any otherstimulus. This supports the role of γδ T-cells as members of innate imune (Guzman et al., 2010).Response of γδ T-cells to stimulation with cytokines is characterized by the release of interferongamma (IFN-γ) (Price et al., 2007). γδ T-cells may also have a role as a „killer“ cells, throughtexpression of granulolysin (Endsley et al., 2004) and perforin (Alvarez et al., 2009).The aim of this diploma thesis was study of lymphocytes and choice of an appropriate method ofstudy of lymphocytes. The study concentrated to subpopulation of γδ T-lymphocytes of bovinemammary gland after stimulation by bacteria Staphylococcus aureus. S. aureus is one of the mostcommon factors causing mastitis in cows (Hogan and Smith, 2003; Bannerman et al., 2004).The experiment was conducted on clinically healthy heifers which are endangered by mastitis in thesame way as dairy cows. Heifers with healthy mammary gland are a basis of succesful bred of dairycows.MATERIAL AND METHODSThe experiment was carried out on five clinically healthy heifers, crossbred Holstein and CzechPied. Heifers were 16 – 18 months old. All of them were free of intramammary infections, asdemonstrated through a bacteriological examination of mammary lavages.Before experimental infection, the mammary glands were treated with phosphate buffered saline(PBS) and control samples were taken. They were taken in the same time intervals as followingsamples of populations of lymphocytes after stimulation with inoculum. The time intervals were: 0hrs – 24 hrs (left-front quarter of mammary gland) – 48 hrs (left-rear) – 72 hrs (right-front) and 168hrs (right-rear).There was used bacterial strain S. aureus Newbould 305 (CCM 6275, Czech Collection ofMicroorganisms, Masaryk University, Brno). The inoculum of S. aureus was prepared by growingthe organism on ram blood agar (BA) medium. It was cultivated under continuous rotation (30rpm/min) for 18 hrs at 37 °C. The stock culture was stored at 4 °C. After dilution of the bacterialsuspension to 800 CFU/ml, the inoculum was adjusted in the syringes. It was used 5 ml of bacterialsuspension. The proportion of lymphocytes in the cell suspension obtained from the lavages wasasessed through flow cytometry (FCM) (FACS Calibur apparatus, Becton Dickinson, CA, USA).Arithmetic means and standard deviations were used to describe the proportions of lymphocytes789 | P age
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Mendel University in BrnoFaculty of
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Editors:Ing. Petr Škarpa, Ph.D., A
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MENDELNET 2013PrefaceThis year´s 2
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MENDELNET 2013EVALUATION OF BARE SO
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MENDELNET 2013DESIGN OF THE EQUIPME
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MENDELNET 2013HOW TO IMPRROVE THE C
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MENDELNET 2013PREDICTION OF TECHNOL
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MENDELNET 2013THE DEVICE FOR MECHAN
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MENDELNET 201317 | P age
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MENDELNET 2013INTRODUCTIONThe proce
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MENDELNET 2013season. Year 2012 had
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MENDELNET 2013CONCLUSIONSThe aim of
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MENDELNET 2013WEED INFESTATION OF W
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MENDELNET 2013Crop sequence in seco
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MENDELNET 2013the soil in the early
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MENDELNET 2013INTRODUCTIONThe speci
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MENDELNET 2013The legend : The infl
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MENDELNET 2013DETERMINATION OF SPEC
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MENDELNET 2013Fig. 1 ASD Hand Held
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MENDELNET 2013Fig. 3 The Spectral R
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MENDELNET 2013CHANGES IN THE CONTEN
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MENDELNET 2013after exposure to abi
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MENDELNET 2013Hoffmann, P. 1988. De
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MENDELNET 2013INTRODUCTIONTradition
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MENDELNET 2013Explanation of abbrev
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MENDELNET 2013CHANGES OF WEED SPECT
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MENDELNET 2013Data collected in vin
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MENDELNET 2013REFERENCESANONYM, 200
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MENDELNET 2013INTRODUCTIONIn the Cz
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MENDELNET 2013Fig. 4: Concentration
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MENDELNET 2013SUGAR BEET YIELD PROD
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MENDELNET 2013RESULT AND DISCUSSION
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MENDELNET 2013and it was 18.41 t ha
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MENDELNET 2013DISTRIBUTION OF BARLE
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MENDELNET 2013RLD (cm.cm -3 )14.012
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MENDELNET 2013confirmed. In a shall
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MENDELNET 2013THE IMPACT OF VERMICO
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MENDELNET 2013fermentation, earthwo
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MENDELNET 2013Tab. 5 The significan
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MENDELNET 2013GUTIÉRREZ-MICELI, F.
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MENDELNET 2013INTRODUCTIONLupin was
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MENDELNET 2013Graph III. Average yi
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MENDELNET 2013Picture I. Demage of
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MENDELNET 2013COMPARISON OF THE ECO
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MENDELNET 2013Tab. 1 Rape varieties
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MENDELNET 2013Kč50 000,00Kč45 000
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MENDELNET 2013INTRODUCTIONSpecific
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MENDELNET 2013As can be seen from t
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MENDELNET 2013EVALUATED GRASS SOD A
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MENDELNET 2013REFERENCESBEARD, J.B.
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MENDELNET 2013INTRODUCTIONSemi-natu
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MENDELNET 2013Influence the intensi
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MENDELNET 2013THE CHANGES IN STAND
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MENDELNET 2013ZPS (%) = 0.5 * Σ |x
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MENDELNET 2013statistically signifi
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MENDELNET 2013INTRODUCTIONIn the fi
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MENDELNET 2013data relatively low.
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MENDELNET 2013LUKAS, V., NEUDERT, L
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MENDELNET 2013INTRODUCTIONNitrogen
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MENDELNET 2013This is evident from
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MENDELNET 2013MONITORING RUSTS OCCU
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MENDELNET 2013Fig. 1 Susceptibility
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MENDELNET 2013Roscher Ch., et al. (
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MENDELNET 2013INTRODUCTIONThe conce
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MENDELNET 2013DAISY and HERMES have
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MENDELNET 2013PALOSUO, T., KERSEBAU
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MENDELNET 2013INTRODUCTIONHops esse
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MENDELNET 2013According to Basařov
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MENDELNET 2013TESTING THE VITALITY
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MENDELNET 2013were compared with fi
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MENDELNET 2013and laboratory germin
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MENDELNET 2013EFFECTS OF SOIL CONDI
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MENDELNET 2013The experiment was co
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MENDELNET 2013SAIGUSA, M., YAMAMOTO
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MENDELNET 2013INTRODUCTIONHis Majes
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MENDELNET 2013Table 3. The cost, re
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MENDELNET 2013EVALUATION OF SYRIAN
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MENDELNET 2013genotypes overcame th
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MENDELNET 2013CONCLUSIONSThe evalua
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MENDELNET 2013INTRODUCTIONThe conte
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MENDELNET 2013Table 4. The effect o
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MENDELNET 2013fertilizer applicatio
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MENDELNET 2013INTRODUCTIONIt´s imp
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MENDELNET 2013demonstrated, that da
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MENDELNET 2013STABLE MICROCLIMATE I
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MENDELNET 2013and draught. The temp
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MENDELNET 2013consequence of consum
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MENDELNET 2013frequency (4 - 20 hea
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MENDELNET 2013INTRODUCTIONRaw milk
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MENDELNET 2013milker's hands, and a
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MENDELNET 2013INTRODUCTIONThe most
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MENDELNET 2013Tab. 1 gives results
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MENDELNET 2013EVALUATION OF THE TRA
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MENDELNET 2013Fig. 1 Development le
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MENDELNET 2013USING ICSI IN DIFFERE
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MENDELNET 2013Oocyte handlingCumula
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MENDELNET 2013CONCLUSIONSThe presen
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MENDELNET 2013IN-VITRO ANTIBACTERIA
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MENDELNET 2013Tab.1. zone of inhibi
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MENDELNET 2013COMPARISON OF LACTOSE
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MENDELNET 2013bound to DNA. Chlorid
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MENDELNET 2013HARDING, F., 1996: Mi
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MENDELNET 2013INTRODUCTIONEuropean
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MENDELNET 2013CONCLUSIONSThe aim of
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MENDELNET 2013INTRODUCTIONFish lipi
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MENDELNET 2013The effect of fish or
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MENDELNET 2013COMPARISON OF GRAZING
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MENDELNET 2013E GQ = Σ(D * FV) / 8
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MENDELNET 2013CONCLUSIONSAccording
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MENDELNET 2013INTRODUCTIONThere are
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MENDELNET 2013thoroughbred horses,
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MENDELNET 2013Tab. 3 Line-based shi
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MENDELNET 2013THE EFFECT OF FEEDING
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MENDELNET 2013Fig. 1 - Digital vill
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MENDELNET 2013KIDD, M. T., et al.,
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MENDELNET 2013INTRODUCTIONIn biomed
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MENDELNET 2013lipid droplets in mat
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MENDELNET 2013t(%)100ABab0MHCMLCVal
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MENDELNET 2013HIRAGA, K., HOSHINO,
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MENDELNET 2013INFLUENCE OF DIETARY
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MENDELNET 2013Tab. 1 Content of ani
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MENDELNET 2013THE EFFECT OF BROILER
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MENDELNET 2013The observation was c
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MENDELNET 2013incidence of contusio
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MENDELNET 2013INTRODUCTIONBreeding
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MENDELNET 2013satisfactory, however
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MENDELNET 2013ROOTWELT, V., REKSEN,
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MENDELNET 2013INTRODUCTIONHucul hor
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MENDELNET 2013• statistically eva
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MENDELNET 2013REFERENCESDUŠEK, J.
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MENDELNET 2013INTRODUCTIONIn the la
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MENDELNET 2013Table 2. Body weight
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MENDELNET 2013INTERACTIONS BETWEEN
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MENDELNET 2013Fig. 3: The effect of
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MENDELNET 2013ŘEZÁČ, P., VIZIOV
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MENDELNET 2013INTRODUCTIONMilk prod
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MENDELNET 2013Values of coefficient
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MENDELNET 2013INTRODUCTIONSoil hydr
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MENDELNET 2013Fig. 1 Mineral nitrog
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MENDELNET 2013DESIGN OF THE EQUIPME
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MENDELNET 2013These probes were pla
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MENDELNET 2013CONCLUSIONSOur experi
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MENDELNET 2013INTRODUCTIONAccelerat
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MENDELNET 2013The maximum capillary
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MENDELNET 2013THE INTENSITY OF WATE
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MENDELNET 2013This paper will addre
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MENDELNET 2013L-factor was calculat
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MENDELNET 2013most fertile agricult
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MENDELNET 2013INTRODUCTIONCarbonate
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MENDELNET 2013correlated only with
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MENDELNET 2013PAN, CH., ZHAO, H., Z
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MENDELNET 2013INTRODUCTIONAgricultu
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MENDELNET 2013CalciumEvaluationCa m
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MENDELNET 2013CarbonatesFig. 7: Con
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MENDELNET 2013ŠKARPA P., 2010: Lab
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MENDELNET 2013INTRODUCTIONIntercrop
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MENDELNET 2013Fig. 2 Production of
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MENDELNET 2013THE POSSIBILITY OF US
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MENDELNET 2013109. Content of carbo
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MENDELNET 2013FALLOPIA JAPONICA HOU
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MENDELNET 2013BARNEY, J. N., et al.
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MENDELNET 2013INTRODUCTIONThis work
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MENDELNET 2013and higher concentrat
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MENDELNET 2013A MATHEMATICAL MODEL
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MENDELNET 2013Coefficients of (1) w
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MENDELNET 2013CONCLUSIONSAlthough t
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MENDELNET 2013THE USE OF COMPOST TO
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MENDELNET 2013Topsoil and subsoil w
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MENDELNET 2013L. Plošek, J. Elbl,
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MENDELNET 2013INTRODUCTIONTests of
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MENDELNET 2013Table 2. Preparation
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MENDELNET 2013ANALYSIS OF SPRING BA
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MENDELNET 2013ET. During the growin
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MENDELNET 2013Evapotranspiration ra
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MENDELNET 2013INTRODUCTIONThe aim o
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MENDELNET 2013SOustava“ - Agromet
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MENDELNET 2013THE HISTORY AND TRADI
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MENDELNET 2013spol. s.r.o. Monitore
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MENDELNET 2013ČESKÝ STATISTICKÝ
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MENDELNET 2013INTRODUCTIONAgricultu
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MENDELNET 2013• Definition of ele
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MENDELNET 2013The results are telli
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MENDELNET 2013DIFFERENCE BETWEEN TH
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MENDELNET 2013Picture 1 Cadastral V
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MENDELNET 2013Fig. 1 Degree of eros
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MENDELNET 2013INTRODUCTIONSoil eros
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MENDELNET 2013stable forms of organ
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MENDELNET 2013land use, pesticides,
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MENDELNET 2013EFFECTS OF UV RADIATI
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MENDELNET 2013Flysch Godulian sands
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MENDELNET 2013CONCLUSIONSUV-B radia
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MENDELNET 2013COMPARISON OF METHODS
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MENDELNET 2013sum of the detected a
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MENDELNET 2013same predictive value
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MENDELNET 2013Section - Rural Devel
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MENDELNET 2013THE IMPACT OF WINTER
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MENDELNET 2013accommodation due to
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MENDELNET 2013THE USE OF SOLAR ENER
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MENDELNET 2013The general data of t
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MENDELNET 2013The photovoltaic powe
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MENDELNET 2013FUNCTION OF AGRICULTU
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MENDELNET 2013Fig. 1. The definitio
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MENDELNET 2013EconomicEconomic• E
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MENDELNET 2013RENEWABLE ENERGY, AS
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MENDELNET 2013After analysing the d
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MENDELNET 2013Rural settlements are
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MENDELNET 2013THE IMPACT OF A ROAD
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MENDELNET 2013Three types of CES we
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MENDELNET 2013stream [9] and Kamenn
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MENDELNET 2013THE PROPOSAL OF LANDS
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MENDELNET 2013MATERIAL AND METHODST
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MENDELNET 2013As for our own resear
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MENDELNET 2013occur more often and
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MENDELNET 2013The arrangements how
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MENDELNET 2013Layers of basic mapsQ
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MENDELNET 2013INTRODUCTIONLand cons
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MENDELNET 2013reforms, all balks, b
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MENDELNET 2013ASSESSMENT OF CHANGES
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MENDELNET 2013meets criteria of sel
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MENDELNET 2013unutilization. Extens
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MENDELNET 2013INTRODUCTIONSince joi
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MENDELNET 2013On the contrary a neg
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MENDELNET 2013THEORETICAL AND METHO
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MENDELNET 2013amount of information
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MENDELNET 2013IMPLEMENTING THE STAT
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MENDELNET 2013practice example can
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MENDELNET 2013elaborating the docum
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MENDELNET 2013SOUTH-MORAVIAN COUNTR
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MENDELNET 2013Tab. 1 Number of devi
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MENDELNET 2013REFERENCES1) ADAMCOV
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MENDELNET 2013INTRODUCTIONDespite o
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MENDELNET 2013RESULTS FROM SWOT ANA
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MENDELNET 2013PRIŠENK, J. and BORE
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MENDELNET 2013INTRODUCTIONIt has be
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MENDELNET 2013THE ECONOMIC AND SOCI
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MENDELNET 2013citizen (the buyer) i
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MENDELNET 2013of South Moravian Reg
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MENDELNET 2013INTRODUCTIONWith the
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MENDELNET 2013Fig.1. Map of present
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MENDELNET 2013Fig.2 Hydric potentia
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MENDELNET 2013EVALUATION OF CURRENT
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MENDELNET 2013through prepared ques
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MENDELNET 2013example; it won the s
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MENDELNET 2013THE DEVELOPMENT AND C
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MENDELNET 2013countries was evaluat
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MENDELNET 2013itself. While impleme
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MENDELNET 2013INTRODUCTIONThe Czech
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MENDELNET 2013Fig. 3 Map of the Cze
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MENDELNET 2013CONCLUSIONSProduction
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MENDELNET 2013INTRODUCTIONLandscape
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MENDELNET 2013The 3-level planning
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MENDELNET 2013THE PROPOSAL OF THE W
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MENDELNET 2013demonstrates that env
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MENDELNET 2013INTRODUCTIONLand cons
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MENDELNET 2013Fig. 2: Reconnaissanc
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MENDELNET 2013MEASUREMENT OF EMISSI
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MENDELNET 2013Tab. 1: The range and
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MENDELNET 2013However, the comparis
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MENDELNET 2013INDICATORS OF THE QUA
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MENDELNET 2013Tab. 1 Index of livin
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MENDELNET 2013CONCLUSIONSResults of
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MENDELNET 2013INTRODUCTIONTourism d
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MENDELNET 2013Disadvantages are rep
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MENDELNET 2013INTRODUCTIONThe therm
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MENDELNET 2013FUSARIUM SPECIES SPEC
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MENDELNET 2013Table 1: Climatic reg
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MENDELNET 2013Figure 3: Frequency o
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MENDELNET 2013IOOS, R. - BELHADJ, S
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MENDELNET 2013MONITORING OF PHTHALA
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MENDELNET 2013RESULTS AND DISCUSSIO
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MENDELNET 2013REFERENCESBRIMER, L.,
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MENDELNET 2013INTRODUCTIONSmall far
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MENDELNET 2013Tab. 4: Counts of mic
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MENDELNET 2013significant, the extr
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MENDELNET 2013THE USING OF NEAR INF
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MENDELNET 2013optical probe by 100
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MENDELNET 2013CONCLUSIONSThe device
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MENDELNET 2013INTRODUCTIONLipids wi
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MENDELNET 2013High acid content of
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MENDELNET 2013DYNAMICS OF CHANGES I
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MENDELNET 2013The barley crop was h
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MENDELNET 2013Fig. 5: B-hordeins (%
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MENDELNET 2013HŘIVNA L., GREGOR T.
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MENDELNET 2013INTRODUCTIONPhthalic
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MENDELNET 2013concentrations of DBP
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MENDELNET 2013YIELD AND QUALITY OF
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MENDELNET 2013variants is shown in
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MENDELNET 2013%22,021,020,019,018,0
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MENDELNET 2013EGGSHELL CRACK DETECT
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MENDELNET 2013It consists of three
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MENDELNET 2013magnitude values from
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MENDELNET 2013ANTIMICROBIAL PROTECT
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MENDELNET 2013filled by one drop of
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MENDELNET 2013TRIPATHI, P. AND N.K.
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MENDELNET 2013INTRODUCTIONWhey or m
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MENDELNET 2013values of these sampl
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MENDELNET 2013The microbiological r
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MENDELNET 2013THE INFLUENCE OF POLY
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MENDELNET 2013verified by the seque
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MENDELNET 2013JUMP D.B., 2008: N-3
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MENDELNET 2013Section - Plant Biolo
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MENDELNET 2013NON-DESTRUCTIVE ANALY
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MENDELNET 2013Graph. 1 Content of f
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MENDELNET 2013CONCLUSIONSThe highes
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MENDELNET 2013INTRODUCTIONThe occur
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MENDELNET 2013-2815 Calculated13897
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MENDELNET 2013EFFECT OF STRIGOLACTO
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MENDELNET 2013For the assessment of
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MENDELNET 2013PAPONOV, I. A. TEALE,
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MENDELNET 2013INTRODUCTIONMilk this
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MENDELNET 2013CitoniceFRAA8400007 8
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MENDELNET 2013DETERMINATION OF OIL
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MENDELNET 2013extraction, the secon
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MENDELNET 2013The blue curve (upper
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MENDELNET 2013INTRODUCTIONThe dynam
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MENDELNET 2013auxin source. These d
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MENDELNET 2013PREDICTION OF TECHNOL
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MENDELNET 2013Total volume of PCR r
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MENDELNET 2013DONG, H., COX, T. S.,
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MENDELNET 2013INTRODUCTIONCereals a
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MENDELNET 2013demonstrated in genot
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MENDELNET 2013INTERACTIVE EFFECTS O
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MENDELNET 2013number) were performe
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MENDELNET 2013CONCLUSIONSElevated [
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MENDELNET 2013INTRODUCTIONAnthocyan
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MENDELNET 2013possible that a copy
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MENDELNET 2013INTRODUCTIONBesides t
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MENDELNET 2013DFR expressionThe exp
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MENDELNET 2013INTRODUCTIONThe under
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MENDELNET 2013Tab. 3 The results of
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MENDELNET 2013REAL-TIME SENSING OF
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MENDELNET 2013Heart preparationFirs
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MENDELNET 2013Li, L., T. L. M. ten
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MENDELNET 2013INTRODUCTIONBirds are
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MENDELNET 2013CONCLUSIONSThis work
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MENDELNET 2013THE EFFECT OF MOLYBDE
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MENDELNET 2013statistical program S
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MENDELNET 2013REFERENCESBERSÉNYI,A
Page 709 and 710:
MENDELNET 2013INTRODUCTIONIn compar
Page 711 and 712:
MENDELNET 2013Flight activity of do
Page 713 and 714:
MENDELNET 2013ARACHNOFAUNA OF TREES
Page 715 and 716:
MENDELNET 2013Fig. 2: Distribution
Page 717 and 718:
MENDELNET 2013MILLER F., 1971: Řá
Page 719 and 720:
MENDELNET 2013INTRODUCTIONThe way o
Page 721 and 722:
MENDELNET 2013Calathus micropterus
Page 723 and 724:
MENDELNET 2013HOW ABOUT EATING THE
Page 725 and 726:
MENDELNET 2013In the first analyse
Page 727 and 728:
MENDELNET 2013MARKOVÁ, Z. a HEJDA,
Page 729 and 730:
MENDELNET 2013INTRODUCTIONAll horse
Page 731 and 732:
MENDELNET 2013phenotypically as dar
Page 733 and 734:
MENDELNET 2013underwhite Gene (uw)U
Page 735 and 736:
MENDELNET 2013INTRODUCTIONMeat qual
Page 737 and 738:
MENDELNET 2013The substitution C878
Page 739 and 740: MENDELNET 2013ICHTHYOLOGICAL SURVEY
Page 741 and 742: MENDELNET 2013RESULT AND DISCUSSION
Page 743 and 744: MENDELNET 2013the mostly balanced f
Page 745 and 746: MENDELNET 2013THE EFFECT OF HEAVY M
Page 747 and 748: MENDELNET 201324 h on a shaker at 4
Page 749 and 750: MENDELNET 2013Elased, K. M., T. S.
Page 751 and 752: MENDELNET 2013INTRODUCTIONRecently,
Page 753 and 754: MENDELNET 2013caudal fin is needed
Page 755 and 756: MENDELNET 20133D CHIP AS A TOOL FOR
Page 757 and 758: MENDELNET 2013Fabrication of 3D mic
Page 759 and 760: MENDELNET 2013CONCLUSIONSWe describ
Page 761 and 762: MENDELNET 2013INTRODUCTIONSeveral f
Page 763 and 764: MENDELNET 2013Tab.3: Absolute and r
Page 765 and 766: MENDELNET 2013loci on Sus scrofa ch
Page 767 and 768: MENDELNET 2013INTRODUCTIONThe dog i
Page 769 and 770: MENDELNET 2013Fig. 1 The negative c
Page 771 and 772: MENDELNET 2013ECOLOGY AND HABITAT P
Page 773 and 774: MENDELNET 2013specimens. Data on th
Page 775 and 776: MENDELNET 2013CONCLUSIONSThe evalua
Page 777 and 778: MENDELNET 2013INTRODUCTIONIt is nec
Page 779 and 780: MENDELNET 2013Fig. 2: Nucleus movin
Page 781 and 782: MENDELNET 2013distance comparing to
Page 783 and 784: MENDELNET 2013INTRODUCTIONThe count
Page 785 and 786: MENDELNET 2013Tab .2LocalityVyšavy
Page 787 and 788: MENDELNET 2013Tab. 4LocalityNosisla
Page 789: MENDELNET 2013REFERENCESBrychta, V.
Page 793 and 794: MENDELNET 2013Fig. 2. Proportion of
Page 795 and 796: MENDELNET 2013THE FOOD OF PERCH (PE
Page 797 and 798: MENDELNET 2013Cladocerans were domi
Page 799 and 800: MENDELNET 2013PETERKA, J., MATĚNA,
Page 801 and 802: MENDELNET 2013INTRODUCTIONFrom a pe
Page 803 and 804: MENDELNET 2013REFERENCESDIENER, S.,
Page 805 and 806: MENDELNET 2013Section - Techniques
Page 807 and 808: MENDELNET 2013EVALUATION OF POLLUTI
Page 809 and 810: MENDELNET 2013relief valve into the
Page 811 and 812: MENDELNET 2013the end of laboratory
Page 813 and 814: MENDELNET 2013INTRODUCTIONAgricultu
Page 815 and 816: MENDELNET 2013CONCLUSIONSFig. 1 Box
Page 817 and 818: MENDELNET 2013CONCEPTUAL SOLUTION O
Page 819 and 820: MENDELNET 2013Chemical parameters:
Page 821 and 822: MENDELNET 2013Focusing on the econo
Page 823 and 824: MENDELNET 2013INTRODUCTIONHigher de
Page 825 and 826: MENDELNET 2013RESULT AND DISCUSSION
Page 827 and 828: MENDELNET 2013REFERENCESBAUER, F.,
Page 829 and 830: MENDELNET 2013INTRODUCTIONToday´s
Page 831 and 832: MENDELNET 2013Fig. 1 - Embedded sys
Page 833 and 834: MENDELNET 2013From the above it is
Page 835 and 836: MENDELNET 2013INTRODUCTIONNowadays,
Page 837 and 838: MENDELNET 2013Fig. 1. The average n
Page 839 and 840: MENDELNET 2013LABORATORY MEASUREMEN
Page 841 and 842:
MENDELNET 2013Electronic thermomete
Page 843 and 844:
MENDELNET 2013Fig. 3 Graph of depen
Page 845 and 846:
MENDELNET 2013INTRODUCTIONThe devel
Page 847 and 848:
MENDELNET 2013Graph no. 2 represent
Page 849 and 850:
MENDELNET 2013ANALYSIS OF MODERN ME
Page 851 and 852:
MENDELNET 2013CMT welding systemCMT
Page 853 and 854:
MENDELNET 2013SampleTab. 2 Overview
Page 855 and 856:
MENDELNET 2013INTRODUCTIONCarbon di
Page 857 and 858:
MENDELNET 2013fan power is performe
Page 859 and 860:
MENDELNET 2013TRACTOR ENGINE MODES
Page 861 and 862:
MENDELNET 2013Technical parameters
Page 863 and 864:
MENDELNET 2013Measurement confirmed
Page 865 and 866:
MENDELNET 2013INTRODUCTIONMain natu
Page 867 and 868:
MENDELNET 2013Fig. 2 - Sub-region t
Page 869 and 870:
MENDELNET 2013DEVELOPMENT OF SEEDER
Page 871 and 872:
MENDELNET 2013RESULTS AND DISCUSSIO
Page 873 and 874:
MENDELNET 2013The depth controlling
Page 875 and 876:
MENDELNET 2013INTRODUCTIONInfluence
Page 877 and 878:
MENDELNET 2013In graph 1 is the sma
Page 879 and 880:
MENDELNET 2013DESIGN OF A MONITORIN
Page 881 and 882:
MENDELNET 2013Selection of such typ
Page 883 and 884:
MENDELNET 2013REFERENCESVITÁZEK, I
Page 885 and 886:
MENDELNET 2013INTRODUCTIONFertilisa
Page 887 and 888:
MENDELNET 2013particle-size distrib
Page 889 and 890:
MENDELNET 2013ŠIMA, T. & DUBEŇOV
Page 891 and 892:
MENDELNET 2013INTRODUCTIONBee hive
Page 893 and 894:
MENDELNET 2013RMSmV1601401201008060
Page 895 and 896:
MENDELNET 2013UTILISATION OF PROGRA
Page 897 and 898:
MENDELNET 2013Ivan (1989) stated th
Page 899 and 900:
MENDELNET 2013Fig. 4 Manoeuvre - ri
Page 901 and 902:
MENDELNET 2013EFFECT OF THE SATELLI
Page 903 and 904:
MENDELNET 2013In both experiments w
Page 905 and 906:
MENDELNET 2013operation 125 measure
Page 907 and 908:
Page 909 and 910:
Page 911 and 912:
MENDELNET 2013INTRODUCTIONTheranost
Page 913 and 914:
MENDELNET 2013Fig. 1 Characterizati
Page 915 and 916:
MENDELNET 2013LIPOSOMES AS DRUG CAR
Page 917 and 918:
MENDELNET 2013microplate in various
Page 919 and 920:
MENDELNET 2013Fig. 3: Differential
Page 921 and 922:
MENDELNET 2013THE ROLE OF NITRIC OX
Page 923 and 924:
MENDELNET 2013one such compound bec
Page 925 and 926:
MENDELNET 2013Kováčik, J., Klejdu
Page 927 and 928:
MENDELNET 2013INTRODUCTIONWater is
Page 929 and 930:
MENDELNET 2013For all the heavy met
Page 931 and 932:
MENDELNET 2013THE USE OF NANOTECHNO
Page 933 and 934:
MENDELNET 2013During the analysis 2
Page 935 and 936:
MENDELNET 2013REFERENCES[1] RANDALL
Page 937 and 938:
MENDELNET 2013INTRODUCTIONQuantum d
Page 939 and 940:
MENDELNET 2013Fig. 2: The staining
Page 941 and 942:
MENDELNET 2013REFERENCESAlgar, W. R
Page 943 and 944:
MENDELNET 2013INTRODUCTIONGunshot r
Page 945 and 946:
MENDELNET 2013correlation is seen b
Page 947 and 948:
MENDELNET 2013ELECTROCHEMICAL DETEC
Page 949 and 950:
MENDELNET 2013Electrochemical detec
Page 951 and 952:
MENDELNET 2013Krizkova, S., I. Fabr
Page 953 and 954:
MENDELNET 2013HavlováL.721 Kadlč
Page 955 and 956:
MENDELNET 2013Procházková B. 48
Page 957:
MENDELNET 2013Name of publication:
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