Untitled - MendelNet 2013 - Mendelova zemědělská a lesnická ...

MENDELNET 2013Table 2. Preparation BLUE EXIT - average cell amount and ±SD Pseudokirchneriella subcapitatain 1 mlControl ±SD 0.025 ml.l-1 ± SD 0.125 ml.l-1 ± SD23.9.2013 0 889 583 43 750 - - - -24.9.2013 24 788 510 13 860 627 083 31 250 53 333 235 28525.9.2013 48 925 000 66 667 754 167 0 845 834 16 66726.9.2013 72 1 363 889 136 987 1 089 584 2 084 732 639 179 25027.9.2013 96 1 993 750 189 583 327 084 10 417 658 334 29 167Table 3. Average cell amount and ±SD Anabaena sp. in 1 mlALGEXITBLUE EXITControl ±SD 0,1 ml.l-1 ±SD 0,125 ml.l-1 ±SD23.9.2013 0 818 750 2 083 - - - -24.9.2013 24 190 972 62 564 274 306 100 695 145 370 9 59425.9.2013 48 243 087 42 330 648 611 280 714 180 208 3 12526.9.2013 72 647 569 352 431 559 722 11 111 37 500 4 16727.9.2013 96 129 775 43 837 73 289 4 837 12 153 1 736CONCLUSIONSInhibition tests were carried out with chosen preparations on cultures of green algaPseudokirchneriella subcapitata and cyanobacteria Anabaena sp. and acute toxicity tests on fish(Danio rerio). Effective concentrations for cyanobacteria and algae extermination and toxic effectson fish were found out. The efficiency of preparation by measuring of density in Bürker chamberunder the microscope with fluorescent was controlled. Percentage inhibition with both preparationsduring 96 hours was counted. At acute toxicity tests on (Danio rerio) we found out, that using ofBLUE EXIT is completely safe, because even with hundredfold concentration no fish died. WithALGEXIT there were 7 dead fish after 48 hours in the highest concentration and the value of LC 50ranged from 1 to 10 ml.l -1 . For value determination of LC 50 is necessary to carry out further testswith narrower range of concentration of given preparation.REFERENCESČSN EN ISO 8692. Jakost vod – Zkouška inhibice růstu sladkovodních zelených řas. Praha: Českýnormalizační institut, 2005, 20 s.FERRÃO-FILHO, A. S., KOZLOWSKY-SUZUKI. B. Cyanotoxins: Bioaccumulation and Effectson Aquatic Animals. Marine Drugs. 2011, roč. 9, č. 12, s. 2729-2772. DOI: 10.3390/md9122729.LAMMER, E., CARR, G. J., WENDLER, K., RAWLINGS, J. M., BELANGER S. E.,BRAUNBECK, T. Is the fish embryo toxicity test (FET) with the zebrafish (Danio rerio) a potentialalternative for the fish acute toxicity test?. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGYC-TOXICOLOGY & PHARMACOLOGY. 2009, roč. 149, č. 2, s. 196-209. ISSN 1532-0456MACHADO, M. D., SOARES, E. V. Development of a short-term assay based on the evaluation ofthe plasma membrane integrity of the alga Pseudokirchneriella subcapitata. Applied Microbiologyand Biotechnology. 2012, roč. 95, č. 4, 1035 – 1042. ISSN 0175-7598PLHALOVÁ, L., MÁCOVÁ, S., DOLEŽELOVÁ, P., MARŠÁLEK, P., SVOBODOVÁ, Z.,PIŠTĚKOVÁ, V., BEDÁŇOVÁ, I., VOSLÁŘOVÁ, E., MODRÁ, H. Comparison of TerbutrynAcute Toxicity to Danio rerio and Poecilia reticulata. Acta Veterinaria Brno. 2010, roč. 79, č. 4, s.593-598. ISSN 0001-7213SVOBODOVÁ, Z. (ed.). Toxikologie vodních živočichů. Praha: Státní zemědělské nakladatelství,1987, 232 s.355 | P age